PBR Antibody (EPR5384)

Product Discontinued

PBR Antibody (EPR5384) Summary

• Immunogen: A synthetic peptide corresponding to residues in C-terminus of human TSPO was used as an immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TSPO
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100-500
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Immunoprecipitation 1:10-100
  • Western Blot 1:10000-50000
• Application Notes: Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24621017)

Reactivity Notes

Mouse cross reactivity tested by western blot and Immunohistochemistry. Rat cross reactivity tested by Western Blot only. Rat reactivity reported in scientific literature (PMID: 24621017)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for PBR Antibody (EPR5384)

• benzodiazepine peripheral binding site
• BZRPDBI
• IBP
• MBRPBS
• mDRC
• Mitochondrial benzodiazepine receptor
• PBR
• PBRBPBS
• PBRS
• peripheral benzodiazepine receptor
• Peripheral-type benzodiazepine receptor
• pk18
• PKBSbenzodiazapine receptor (peripheral)
• PTBR
• translocator protein (18kDa)
• translocator protein
• TSPO

Background

TSPO (Mitochondrial benzodiazepine receptor) is present mainly in the mitochondrial compartment of peripheral tissues, and it interacts with some benzodiazepines and has different affinities than its endogenous counterpart. This protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PBR Antibody (NBP1-95674)(13)

Publications using NBP1-95674

• Porret E, Kereselidze D, Dauba A et al. Refining the delivery and therapeutic efficacy of cetuximab using focused ultrasound in a mouse model of glioblastoma: an 89Zr-cetuximab immunoPET study European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2022-12-15 [PMID: 36529256] (IHC-Fr, Mouse)
• Backhaus P, Roll W, Beuker C et al. Initial experience with [18F]DPA-714 TSPO-PET to image inflammation in primary angiitis of the central nervous system Eur. J. Nucl. Med. Mol. Imaging 2020-01-20 [PMID: 31960097]
• Klein B, Mrowetz H, Barker CM et al. Age Influences Microglial Activation After Cuprizone-Induced Demyelination. Front Aging Neurosci. 2018-09-20 [PMID: 30297998] (IF/IHC, Mouse)
• Hellberg S, Liljenback H, Eskola O et al. Positron Emission Tomography Imaging of Macrophages in Atherosclerosis with 18F-GE-180, a Radiotracer for Translocator Protein (TSPO) Contrast Media Mol Imaging 2018-06-29 [PMID: 29950954]
• Calevro A, Cotel MC, Natesan S et al. Effects of chronic antipsychotic drug exposure on the expression of Translocator Protein and inflammatory markers in rat adipose tissue Psychoneuroendocrinology 2018-05-16 [PMID: 29793094] (Rat)
• Zinnhardt B, Pigeon H, Theze B et al. Combined PET imaging of the inflammatory tumor microenvironment identifies margins of unique radiotracer uptake. Cancer Res. 2017-01-30 [PMID: 28137769] (IHC-P, Mouse)
• Domene A, Cavanagh C, Page G et al. Expression of Phenotypic Astrocyte Marker Is Increased in a Transgenic Mouse Model of Alzheimer's Disease versus Age-Matched Controls: A Presymptomatic Stage Study. Int J Alzheimers Dis. 2016-09-27 [PMID: 27672476] (Mouse)
• Hellberg S, Silvola JM, Kiugel M et al. 18-kDa translocator protein ligand 18F-FEMPA: Biodistribution and uptake into atherosclerotic plaques in mice. J Nucl Cardiol. 2016-05-25 [PMID: 27225517] (IF/IHC, Mouse)
• Gargiulo S, Anzilotti S, Coda AR et al. Imaging of brain TSPO expression in a mouse model of amyotrophic lateral sclerosis with (18)F-DPA-714 and micro-PET/CT. Eur. J. Nucl. Med. Mol. Imaging. 2016-01-27 [PMID: 26816193] (IHC-Fr, Mouse)
• Zinnhardt B, Viel T, Wachsmuth L et al. Multimodal imaging reveals temporal and spatial microglia and matrix metalloproteinase activity after experimental stroke J. Cereb. Blood Flow Metab. 2015-07-01 [PMID: 26126867] (IHC-P, Mouse)
• Serriere S, Tauber C, Vercouillie J et al. Amyloid load and translocator protein 18 kDa in APPswePS1-dE9 mice: a longitudinal study Neurobiol. Aging. 2015-01-16 [PMID: 25680265] (WB, IHC-Fr, Mouse)
• Bae KR, Shim HJ, Balu D et al. Translocator Protein 18 kDa Negatively Regulates Inflammation in Microglia. J Neuroimmune Pharmacol 2014-04-01 [PMID: 24687172] (ICC/IF, WB, IHC-Fr, Mouse)
• Pottier G, Bernards N, Dolle F, Boisgard R. [18F]DPA-714 as a biomarker for positron emission tomography imaging of rheumatoid arthritis in an animal model. Arthritis Res. Ther. 2014-03-13 [PMID: 24621017] (IHC-Fr, Rat)

FAQs for PBR Antibody (NBP1-95674). (Showing 1 - 1 of 1 FAQs).

1. Is This antibody certified to work in mice using formalin-fixed, paraffin embedded tissue at a concentration of 1:500?
   • The suggested starting dilution when performing IHC-P with this antibody is 1:100-1:250, however we always recommend that customers optimise the antibody dilution and staining conditions to suit their particular experimental set up.

Bioinformatics

• Gene Symbol: TSPO
• Entrez:
  • 706(Human)
  • 12257(Mouse)
  • 24230(Rat)
• Uniprot:
  • P30536()