Recombinant Rat PBEF/Visfatin/NAMPT FLAG Protein

• Reactivity: Rt
• Applications: PAGE
• Concentration: 0.35 mg/ml

Recombinant Rat PBEF/Visfatin/NAMPT FLAG Protein Summary

• Description: Rat PBEF/Visfatin/NAMPT (visfatin/PBEF) (aa 1-491) fused at the N-terminus to a FLAG(R)-tag.
  
  Source: HEK 293 cells

  Uniprot ID: Q80Z29

• Source: HEK293
• Protein/Peptide Type: Recombinant Protein
• Gene: NAMPT
• Purity: >90% SDS-PAGE
• Endotoxin Note: <0.1EU/ug purified protein (LAL test; Lonza).

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Liquid. 0.2um-filtered solution in PBS, pH 7.2
• Preservative: No Preservative
• Concentration: 0.35 mg/ml
• Purity: >90% SDS-PAGE

Alternate Names for Recombinant Rat PBEF/Visfatin/NAMPT FLAG Protein

• EC 2.4.2.12
• MGC117256
• NAmPRTase
• NAMPT
• nicotinamide phosphoribosyltransferase
• NMPRTase
• PBEF
• PBEF1
• PBEF1110035O14Rik
• PBEF1DKFZp666B131
• Pre-B cell-enhancing factor
• pre-B-cell colony enhancing factor 1
• Pre-B-cell colony-enhancing factor 1
• VF
• Visfatin

Background

NAMPT encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NAMPT
• Uniprot:
  • Q80Z29()