Recombinant Human PBEF/Visfatin/NAMPT Protein

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SDS-Page: Recombinant Human PBEF/Visfatin/NAMPT Protein [NBP2-76226] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.
Bioactivity: Recombinant Human PBEF/Visfatin/NAMPT Protein [NBP2-76226] - PBEF/Visfatin/NAMPT induced expression of IL-6 from human PBMCs.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
LYOPH

Order Details

Recombinant Human PBEF/Visfatin/NAMPT Protein Summary

Description
An un-tagged bioactive recombinant protein corresponding to Human PBEF/Visfatin/NAMPT

Source: E. coli

Amino Acid Sequence: MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH

Specificity
PBEF/Visfatin/NAMPT is a monomer, 52.7 kDa (466 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Details of Functionality
Bioactive protein but no acceptance criteria limits established
Protein/Peptide Type
Recombinant Protein
Gene
NAMPT
Purity
>90%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Theoretical MW
52.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Preservative
No Preservative
Concentration
LYOPH
Purity
>90%, by SDS-PAGE
Reconstitution Instructions
Sterile 20 mM HCI at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Human PBEF/Visfatin/NAMPT Protein

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for PBEF/Visfatin/NAMPT Recombinant Protein (NBP2-76226) (0)

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Bioinformatics

Gene Symbol NAMPT