Recombinant Human PBEF/Visfatin/NAMPT Protein

Product Discontinued

Recombinant Human PBEF/Visfatin/NAMPT Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-491 of Human PBEF/Visfatin/NAMPT
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MNPAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKLR KVKYEETVFY GLQYILNKYL KGKVVTKEKI QEAKDVYKEH FQDDVFNEKG WNYILEKYDG HLPIEIKAVP EGFVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGLALIKKY YGTKDPVPGY SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV SRSTQAPLII RPDSGNPLDT VLKVLEILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT LQEIVEGMKQ KMWSIENIAF GSGGGLLQKL TRDLLNCSFK CSYVVTNGLG INVFKDPVAD PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGQDLLHT VFKNGKVTKS YSFDEIRKNA QLNIELEAAH H

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NAMPT
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris buffer (pH 8.0), 0.1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human PBEF/Visfatin/NAMPT Protein

• EC 2.4.2.12
• MGC117256
• NAmPRTase
• NAMPT
• nicotinamide phosphoribosyltransferase
• NMPRTase
• PBEF
• PBEF1
• PBEF1110035O14Rik
• PBEF1DKFZp666B131
• Pre-B cell-enhancing factor
• pre-B-cell colony enhancing factor 1
• Pre-B-cell colony-enhancing factor 1
• VF
• Visfatin

Background

Visfatin is pre-B cell colony-enhancing factor 1(PBEF), which is predominantly secreted from visceral adipose tissue both in humans and mice. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis, or a visceral-fat derived hormone that acts by binding and activating the insulin receptor. Recombinant human visfatin, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for PBEF/Visfatin/NAMPT Recombinant Protein (NBC1-18484)(1)

Publication using NBC1-18484

• Nishimuta JF, Levenston ME. Meniscus is more susceptible than cartilage to catabolic and anti-anabolic effects of adipokines Osteoarthr. Cartil. 2015-04-23 [PMID: 25917638]

Bioinformatics

• Gene Symbol: NAMPT