Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP3-11683] - Sample standard curve

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell Lysate, Cell-based assays
• Standard Curve Range: 0.25-16 ng/ml
• Sensitivity: 30 pg/ml

Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) Summary

• Description: The Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) is to be used for the in vitro quantitative determination of human PBEF/Visfatin/NAMPT in cell lysates or cell-based assays (screening).
• Specificity: Detects human Nampt. Weakly cross-reacts (<15%) with mouse Nampt and rat Nampt. Does not cross-react with human adiponectin, human resistin, human RELM-beta or human leptin.
• Standard Curve Range: 0.25-16 ng/ml
• Sensitivity: 30 pg/ml
• Assay Type: Sandwich ELISA (quantitative)
• Inter-Assay: Sample1: CV = 4.02% (n=6) Sample1: CV = 7.40% (n=6)
• Intra-Assay: Sample1: CV = 2.73% (n=10) Sample1: CV = 9.76% (n=10)
• Spike Recovery: 90-105%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NAMPT

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

• EC 2.4.2.12
• MGC117256
• NAmPRTase
• NAMPT
• nicotinamide phosphoribosyltransferase
• NMPRTase
• PBEF
• PBEF1
• PBEF1110035O14Rik
• PBEF1DKFZp666B131
• Pre-B cell-enhancing factor
• pre-B-cell colony enhancing factor 1
• Pre-B-cell colony-enhancing factor 1
• VF
• Visfatin

Background

NAMPT encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for PBEF/Visfatin/NAMPT ELISA Kit (NBP3-11683). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NAMPT