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Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

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ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP3-11683] - Sample standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell Lysate, Cell-based assays
Standard Curve Range
0.25-16 ng/ml
Sensitivity
30 pg/ml

Order Details

Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) Summary

Description
The Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) is to be used for the in vitro quantitative determination of human PBEF/Visfatin/NAMPT in cell lysates or cell-based assays (screening).
Specificity
Detects human Nampt. Weakly cross-reacts (<15%) with mouse Nampt and rat Nampt. Does not cross-react with human adiponectin, human resistin, human RELM-beta or human leptin.
Standard Curve Range
0.25-16 ng/ml
Sensitivity
30 pg/ml
Assay Type
Sandwich ELISA (quantitative)
Inter-Assay
Sample1: CV = 4.02% (n=6) Sample1: CV = 7.40% (n=6)
Intra-Assay
Sample1: CV = 2.73% (n=10) Sample1: CV = 9.76% (n=10)
Spike Recovery
90-105%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
NAMPT

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 1 bottle Lysis Buffer 10X (12 ml)
  2. 1 bottle Stop Solution (12 ml)
  3. 1 bottle TMB Substrate Solution (12 ml)
  4. 1 plate coated with human PBEF/Visfatin/NAMPT Antibody (6 x 16-well strips)
  5. 1 vial Detection Antibody (60 ul)
  6. 1 vial HRP 100X (HRP Conjugated anti-rabbit IgG) (150 ul)
  7. 1 vial human PBEF/Visfatin/NAMPT Standard (lyophilized) (32 ng)
  8. 2 bottles ELISA Buffer 10X (2 x 30 ml)
  9. 2 bottles Wash Buffer 10X (2 x 30 ml)
  10. 2 plate sealers (plastic film)
  11. 2 silica Gel Minibags

Alternate Names for Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Background

NAMPT encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol NAMPT