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Mouse PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

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ELISA: Mouse PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76711] - Samples were spiked with high concentrations of Mouse PBEF/Visfatin/NAMPT and diluted with Reference Standard & Sample Diluent to produce samples ...read more
ELISA: Mouse PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76711] - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.625 - 40ng/mL
Sensitivity
0.375ng/mL

Order Details

Mouse PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.625 - 40ng/mL
Sensitivity
0.375ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.83
Intra-Assay
CV% < 4.99
Spike Recovery
88-109%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
NAMPT

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Mouse PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Background

NAMPT encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol NAMPT