New pricing — Effective July 1, 2022 -

Amidst rising costs across all areas of our business, and aggressive attempts to implement cost savings without sacrificing quality, we announce the need to implement a cost increase starting July 1, 2022. If you have questions, please contact your sales representative.

Home » PBEF/Visfatin/NAMPT » PBEF/Visfatin/NAMPT ELISA Kits » Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

 Submit a Review

Images

 
(
Enlarge)
ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76710] - Standard Curve Reference
ELISA: Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) [NBP2-76710] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
0.313 - 20ng/mL
Sensitivity
0.188ng/mL

Order Details

100% Guarantee on every product

Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.313 - 20ng/mL
Sensitivity
0.188ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.31
Intra-Assay
CV% < 4.96
Spike Recovery
87-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
NAMPT

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100x)
  4. Concentrated HRP Conjugate (100x)
  5. Concentrated Wash Buffer (25x)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate (Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Insulin was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(14 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Adiponectin/Acrp30
[HRP]
DRP300
(6 Reviews)
(150 Publications)
Species: Hu
Applications: ELISA
IL-6 [HRP]
D6050
(32 Reviews)
(605 Publications)
Species: Hu
Applications: ELISA
Leptin/OB [HRP]
DLP00
(8 Reviews)
(128 Publications)
Species: Hu
Applications: ELISA
Resistin [HRP]
DRSN00
(1 Review)
(31 Publications)
Species: Hu
Applications: ELISA
TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100&amp;nbsp;pg/mL.
(36 Reviews)
(708 Publications)
Species: Hu
Applications: BA
Sirtuin 1/SIRT1
Antibody (1F3) - BSA ...
NBP1-51641
Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.
Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).
Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
(7 Publications)
Species: Hu, Mu, Pm, Rb, Rt
Applications: ELISA, Flow-IC, Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, WB
C-Reactive
Protein/CRP [HRP]
DCRP00
(4 Reviews)
(117 Publications)
Species: Hu
Applications: ELISA
PPAR gamma/NR1C3
Antibody - BSA Free
NBP2-22106
Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
(2 Reviews)
(6 Publications)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Insulin Receptor
beta Antibody
NBP2-12793
Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500. Image submitted by a verified customer review.
Immunoprecipitation: Insulin Receptor beta Antibody [NBP2-12793] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
Apelin Antibody
NBP2-31176
Immunohistochemistry-Paraffin: Apelin Antibody [NBP2-31176] - Staining of human isolated adipocytes. Antibody dilution 1:2000.
(3 Publications)
Species: Hu, Mu, Po, Sh
Applications: ELISA, IHC, IHC-P, WB
Serpin A12 [Biotin]
DY4410
(1 Review)
(1 Publication)
Species: Hu
Applications: ELISA
ERK2 Antibody
(SZ25-01)
NBP2-67360
Flow Cytometry: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
(1 Review)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB

RBP4/Retinol-Binding P
DRB400
(18 Publications)
Species: Hu
Applications: ELISA
ERK1 Antibody (1E5)
NBP2-22203
Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Recombinant Human IL-8/CXCL8 (Catalog&amp;nbsp;# 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog&amp;nbsp;# AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20&amp;nbsp;ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog&amp;nbsp;# MAB208). The ND50 is typically0.08-0.4&amp;nbsp;µg/mL.
IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
(10 Reviews)
(104 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
CCL2/JE/MCP-1 [HRP]
DCP00
(8 Reviews)
(214 Publications)
Species: Hu
Applications: ELISA
CSRP1 Antibody
AF5739
Western blot shows lysates of ME-180 human cervical epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5&amp;nbsp;µg/mL Goat Anti-Human/Mouse/Rat CSRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). For additional reference, recombinant human CSRP1, CSRP2, CSRP3 (5&amp;nbsp;ng/lane) were included. A specific band for CSRP1 was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu, Mu, Rt
Applications: WB
AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100&amp;nbsp;ng/mL Human&amp;nbsp;PDGF&amp;nbsp;(Catalog&amp;nbsp;# 120‑HD) for 15&amp;nbsp;minutes and MCF‑7 human breast cancer cell line untreated or treated with 100&amp;nbsp;ng/mL&amp;nbsp;Recombinant Human IGF-1 (Catalog&amp;nbsp;# 291‑G1) for 15&amp;nbsp;minutes. PVDF membrane was probed with 0.5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group&amp;nbsp;1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog&amp;nbsp;# 291-G1) for 15&amp;nbsp;minutes, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887). This experiment was conducted&amp;nbsp;under&amp;nbsp;reducing&amp;nbsp;conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with&amp;nbsp;100&amp;nbsp;ng/mL Recombinant human IGF-1 (Catalog&amp;nbsp;# 291-G1, dark orange filled histogram) for 15&amp;nbsp;minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog&amp;nbsp;# AF887) or control antibody (Catalog&amp;nbsp;# AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog&amp;nbsp;# F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
(26 Publications)
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
Human
PBEF/Visfatin/NAMPT E ...
NBP2-76710
Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)
Species: Hu
Applications: ELISA

Publications for PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710) (0)

There are no publications for PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710) (0)

There are no reviews for PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PBEF/Visfatin/NAMPT Products

Bioinformatics Tool for PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710)

Discover related pathways, diseases and genes to PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710)

Discover more about diseases related to PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710).
 

Pathways for PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710)

View related products by pathway.

PTMs for PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710)

Learn more about PTMs related to PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710).
 

Research Areas for PBEF/Visfatin/NAMPT ELISA Kit (NBP2-76710)

Find related products by research area.

Blogs on PBEF/Visfatin/NAMPT

There are no specific blogs for PBEF/Visfatin/NAMPT, but you can read our latest blog posts.

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005
IL-6 [HRP]
IL-6 [HRP]
D6050

Contact Information

Product PDFs

  Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Human PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) and receive a gift card or discount.


Bioinformatics

Gene Symbol NAMPT