Human, Mouse, Rat PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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    • Catalog Number
      KA1865
    • Availability
      Product Discontinued

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Human, Mouse, Rat PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric) Summary

Description
Quality control test: Standard Curve. NAMPT/Nampt (Human Mouse Rat) ELISA Kit is an in vitro quantitative assay for detecting NAMPT/Nampt based on the principle of Competitive Enzyme Immunoassay.
Standard Curve Range
0.1 to 1000 ng/mL
Sensitivity
379 pg/mL
Assay Type
Competitive ELISA
Inter-Assay
CV% <15%
Intra-Assay
CV% < 10%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
NAMPT

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Useful for quantification.
Publications
Read Publication using KA1865.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Anti-Visfatin polyclonal antibody (Item N)
  2. Assay Diluent B (Item E)
  3. Biotinylated Visfatin Peptide (Item F)
  4. HRP-Streptavidin Concentrate (Item G)
  5. Positive control (Item M)
  6. Standard Visfatin Peptide (Item C)
  7. Stop Solution (Item I)
  8. TMB One-Step Substrate Reagent (Item H)
  9. Visfatin Microplate (Item A)
  10. Wash Buffer Concentrate (20x) (Item B)

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human, Mouse, Rat PBEF/Visfatin/NAMPT ELISA Kit (Colorimetric)

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Background

NAMPT encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for PBEF/Visfatin/NAMPT ELISA Kit (KA1865)(1)

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Product General Protocols

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FAQs for PBEF/Visfatin/NAMPT ELISA Kit (KA1865). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol NAMPT