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PBEF/Visfatin/NAMPT Antibody

 (2 Publications)
 (1 Review)
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Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - DETECTS A STRONG BAND AROUND 37Kda AND ALSO THE OTHER ISOFORMS BETWEEN 40 AND 55Kda. THE DILUTION OF THE ANTIBODY CAN BE ENHANCED 1:5000. Image from a verified customer review.
Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - DETECTS A STRONG BAND AROUND 37Kda AND ALSO THE OTHER ISOFORMS BETWEEN 40 AND 55Kda. THE DILUTION OF THE ANTIBODY CAN BE ENHANCED 1:5000. Image from a verified ...read more
Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - NAMPT Antibody [NBP2-23795] - Analysis of NAMPT in human tumor cell line INSTA-Blot at 1:1000. Lanes: (A) HeLa, (B) Jurkat, (C) Daudi, (D) HEK 293, (E) Rh30, (F) ...read more
Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - NAMPT Antibody [NBP2-23795] - Western blot analysis of NAMPT in multiple samples using NBP2-23795 at 1:1000. Lanes: (1) Human spleen, (2) Human spleen in the ...read more

Product Details

Summary
Reactivity Hu, Rt, Mu, Po, Bv, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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PBEF/Visfatin/NAMPT Antibody Summary

Immunogen
Amino acid 395-412 FKDPVADPNKRSKKGRLS of human NAMPT protein were used as the immunogen.
Predicted Species
Mouse (100%), Porcine (100%), Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAMPT
Purity
Unpurified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1: 1000-1:2000
Application Notes
WB reported in a verified customer review.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-23795 in the following application:
Publications
Read Publications using
NBP2-23795 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Crude whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for PBEF/Visfatin/NAMPT Antibody

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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PBEF/Visfatin/NAMPT An
NBP2-23795
PBEF/Visfatin/NAMPT Antibody
Species: Hu, Rt, Mu, Po, Bv, Pm
Applications: WB
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Publications for PBEF/Visfatin/NAMPT Antibody (NBP2-23795)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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Review for PBEF/Visfatin/NAMPT Antibody (NBP2-23795) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-23795:
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Images Ratings Applications Species Date Details
Western Blot PBEF/Visfatin/NAMPT NBP2-23795
Enlarge 		5
reviewed by:
Prabir Chakraborty
 WB Human 01/24/2014
View

Summary

ApplicationWestern Blot
Sample TestedHEK293T, A2780
SpeciesHuman
Lot0963034A
CommentsTHIS NAMPT ANTIBODY DETECTS A STRONG BAND AROUND 37Kda AND ALSO THE OTHER ISOFORMS BETWEEN 40 AND 55Kda. THE DILUTION OF THE ANTIBODY CAN BE ENHANCED TO 1:5000.

Blocking

Blocking Details4% BSA IN TBST

Primary Anitbody

Dilution Ratio1:2000 IN 4%BSA IN TBST, INCUBATED O/N AT 4*C

Secondary Antibody

Secondary DescriptionGOAT ANTI-RABBIT IgG-HRP
Secondary Manufacturer Cat#sc-2301
Secondary Concentration1:10,000

Details

Detection NotesECL

Comments

CommentsTHIS NAMPT ANTIBODY DETECTS A STRONG BAND AROUND 37Kda AND ALSO THE OTHER ISOFORMS BETWEEN 40 AND 55Kda. THE DILUTION OF THE ANTIBODY CAN BE ENHANCED TO 1:5000.

Product General Protocols

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FAQs for PBEF/Visfatin/NAMPT Antibody (NBP2-23795). (Showing 1 - 1 of 1 FAQs).

  1. I have a question about antibody NBP2-23795 anti-NAMPT. I would like to know how much protein is loaded per lane on the Western blot shown on the datasheet. Would you have this information available that you could share?
    • Approximately 30 ug/protein was loaded per lane of a 12 well SDS-mini gel.

Secondary Antibodies

 

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Diseases for PBEF/Visfatin/NAMPT Antibody (NBP2-23795)

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Western Blot PBEF/Visfatin/NAMPT NBP2-23795
Enlarge
 Prabir Chakraborty
01/24/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol NAMPT
Uniprot