PBEF/Visfatin/NAMPT Antibody

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - DETECTS A STRONG BAND AROUND 37Kda AND ALSO THE OTHER ISOFORMS BETWEEN 40 AND 55Kda. THE DILUTION OF THE ANTIBODY CAN BE ENHANCED 1:5000. Image from a verified customer review.

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - NAMPT Antibody [NBP2-23795] - Analysis of NAMPT in human tumor cell line INSTA-Blot at 1:1000. Lanes: (A) HeLa, (B) Jurkat, (C) Daudi, (D) HEK 293, (E) Rh30, (F) A375, (G) T98G, (H) HCT-116, (I) Hep-2.

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - NAMPT Antibody [NBP2-23795] - Western blot analysis of NAMPT in multiple samples using NBP2-23795 at 1:1000. Lanes: (1) Human spleen, (2) Human spleen in the presence of immunizing/blocking peptide, (3) HeLa, (4) Jurkat, (5) Rh30, (6) T98G, (7) HCT-116.

Product Discontinued

PBEF/Visfatin/NAMPT Antibody Summary

• Immunogen: Amino acid 395-412 FKDPVADPNKRSKKGRLS of human NAMPT protein were used as the immunogen.
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAMPT
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1: 1000-1:2000
• Application Notes: WB reported in a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Crude whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for PBEF/Visfatin/NAMPT Antibody

• EC 2.4.2.12
• MGC117256
• NAmPRTase
• NAMPT
• nicotinamide phosphoribosyltransferase
• NMPRTase
• PBEF
• PBEF1
• PBEF1110035O14Rik
• PBEF1DKFZp666B131
• Pre-B cell-enhancing factor
• pre-B-cell colony enhancing factor 1
• Pre-B-cell colony-enhancing factor 1
• VF
• Visfatin

Background

NAMPT encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PBEF/Visfatin/NAMPT Antibody (NBP2-23795)(2)

Publications using NBP2-23795

• Ribas-Latre A, Baselga-Escudero L, Casanova E et al. Dietary proanthocyanidins modulate BMAL1 acetylation, Nampt expression and NAD levels in rat liver Sci Rep. 2015-06-08 [PMID: 26051626] (WB, Rat)
  
  Details:
  Nampt antibody (Imgenex IMX-6096) was used at 1:1000 dilution in WB for the detection of nicotinamide phosphoribosyltransferase /Nampt in Rat liver (see full text for protocol details, data image shown in Supplementary Figure 1).
• Ribas-Latre A, Baselga-Escudero L, Casanova E et al. Chronic consumption of dietary proanthocyanidins modulates peripheral clocks in healthy and obese rats J. nutr. Biochem. 4192-01-01 [PMID: 25459887] (WB, Rat)
  
  Details:
  PBEF/Visfatin/nAMPT antibody used at 1:1000 dilution in WB on lysates of liver (A) and mesenteric white adipose tissue /mWAT (B) of rats chronically supplemented with different doses of grape seed proanthocyanidin extracts/GSPE for 3 weeks (Fig. 4). [note:- WB image is not shown Dr Arola's team acquired polyclonal antibody from Imgenex, Monoclonal appears to be a typo in Western blot analyses section of Materials and Methods in this paper].

Review for PBEF/Visfatin/NAMPT Antibody (NBP2-23795) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Prabir Chakraborty WB Human 01/24/2014

Summary

• Application: Western Blot
• Sample Tested: HEK293T, A2780
• Species: Human
• Lot: 0963034A

FAQs for PBEF/Visfatin/NAMPT Antibody (NBP2-23795). (Showing 1 - 1 of 1 FAQs).

1. I have a question about antibody NBP2-23795 anti-NAMPT. I would like to know how much protein is loaded per lane on the Western blot shown on the datasheet. Would you have this information available that you could share?
   • Approximately 30 ug/protein was loaded per lane of a 12 well SDS-mini gel.

Bioinformatics

• Gene Symbol: NAMPT
• Uniprot:
  • P43490()