PBEF/Visfatin/NAMPT Antibody

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Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - DETECTS A STRONG BAND AROUND 37Kda AND ALSO THE OTHER ISOFORMS BETWEEN 40 AND 55Kda. THE DILUTION OF THE ANTIBODY CAN BE ENHANCED 1:5000. Image from a verified ...read more
Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - NAMPT Antibody [NBP2-23795] - Analysis of NAMPT in human tumor cell line INSTA-Blot at 1:1000. Lanes: (A) HeLa, (B) Jurkat, (C) Daudi, (D) HEK 293, (E) Rh30, (F) ...read more
Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23795] - NAMPT Antibody [NBP2-23795] - Western blot analysis of NAMPT in multiple samples using NBP2-23795 at 1:1000. Lanes: (1) Human spleen, (2) Human spleen in the ...read more

Product Details

Summary
Reactivity Hu, Rt, Mu, Po, Bv, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PBEF/Visfatin/NAMPT Antibody Summary

Immunogen
Amino acid 395-412 FKDPVADPNKRSKKGRLS of human NAMPT protein were used as the immunogen.
Predicted Species
Mouse (100%), Porcine (100%), Bovine (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAMPT
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1: 1000-1:2000
Application Notes
WB reported in a verified customer review.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-23795 in the following application:

Publications
Read Publications using
NBP2-23795 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Crude whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for PBEF/Visfatin/NAMPT Antibody

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PBEF/Visfatin/NAMPT Antibody (NBP2-23795)(2)

Review for PBEF/Visfatin/NAMPT Antibody (NBP2-23795) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-23795:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot PBEF/Visfatin/NAMPT NBP2-23795
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reviewed by:
Prabir Chakraborty
WB Human 01/24/2014
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Summary

ApplicationWestern Blot
Sample TestedHEK293T, A2780
SpeciesHuman
Lot0963034A
CommentsTHIS NAMPT ANTIBODY DETECTS A STRONG BAND AROUND 37Kda AND ALSO THE OTHER ISOFORMS BETWEEN 40 AND 55Kda. THE DILUTION OF THE ANTIBODY CAN BE ENHANCED TO 1:5000.

Blocking

Blocking Details4% BSA IN TBST

Primary Anitbody

Dilution Ratio1:2000 IN 4%BSA IN TBST, INCUBATED O/N AT 4*C

Secondary Antibody

Secondary DescriptionGOAT ANTI-RABBIT IgG-HRP
Secondary Manufacturer Cat#sc-2301
Secondary Concentration1:10,000

Details

Detection NotesECL

Comments

CommentsTHIS NAMPT ANTIBODY DETECTS A STRONG BAND AROUND 37Kda AND ALSO THE OTHER ISOFORMS BETWEEN 40 AND 55Kda. THE DILUTION OF THE ANTIBODY CAN BE ENHANCED TO 1:5000.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PBEF/Visfatin/NAMPT Antibody (NBP2-23795). (Showing 1 - 1 of 1 FAQs).

  1. I have a question about antibody NBP2-23795 anti-NAMPT. I would like to know how much protein is loaded per lane on the Western blot shown on the datasheet. Would you have this information available that you could share?
    • Approximately 30 ug/protein was loaded per lane of a 12 well SDS-mini gel.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for PBEF/Visfatin/NAMPT Antibody (NBP2-23795)

Discover related pathways, diseases and genes to PBEF/Visfatin/NAMPT Antibody (NBP2-23795). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PBEF/Visfatin/NAMPT Antibody (NBP2-23795)

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Pathways for PBEF/Visfatin/NAMPT Antibody (NBP2-23795)

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PTMs for PBEF/Visfatin/NAMPT Antibody (NBP2-23795)

Learn more about PTMs related to PBEF/Visfatin/NAMPT Antibody (NBP2-23795).
 

Research Areas for PBEF/Visfatin/NAMPT Antibody (NBP2-23795)

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Recent Reviews

5
5
1
4
0
3
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2
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1
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Prabir Chakraborty
01/24/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NAMPT
Uniprot