PBEF/Visfatin/NAMPT Antibody

Western Blot: PBEF/Visfatin/NAMPT Antibody [NBP2-23667] - Analysis of NAMPT in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lane 2 shows human, Lane 3 shows mouse and Lane 4 shows rat skeletal muscle using NBP2-23667 at 1:5000 dilution.

Product Discontinued

PBEF/Visfatin/NAMPT Antibody Summary

• Immunogen: A portion of amino acid 30-80 of human NAMPT/Visfatin was used as the immunogen.
• Predicted Species: Bovine (94%), Equine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAMPT
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 - 1:5000

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: zebrafish (83%). The NAMPT protein has multiple variants of varying molecular weights including ~55.5 kDa (alternative variant a), 41.9 kDa (alternative variant b), 35.6 kDa alternative variant c), 28.5 kDa (alternative variant d), and 26.8 (alternative variant e). Researchers are encouraged to see Aceview (http://www.ncbi.nlm.nih.gov/ieb/research/acembly/av.cgi?db=human&term=nampt&submit=Go) for additional information about NAMPT forms. NBP2-23795 detects an ~38 kDa band in western blot in the cells and tissues screened. This band may correspond to NAMPT, alternative variants b or c, or another NAMPT form.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Crude whole rabbit sera with 50% Glycerol
• Preservative: 0.025% Sodium Azide
• Purity: Unpurified

Alternate Names for PBEF/Visfatin/NAMPT Antibody

• EC 2.4.2.12
• MGC117256
• NAmPRTase
• NAMPT
• nicotinamide phosphoribosyltransferase
• NMPRTase
• PBEF
• PBEF1
• PBEF1110035O14Rik
• PBEF1DKFZp666B131
• Pre-B cell-enhancing factor
• pre-B-cell colony enhancing factor 1
• Pre-B-cell colony-enhancing factor 1
• VF
• Visfatin

Background

NAMPT is a 491 amino acid adipokine belonging to the NAPRTase family. It promotes angiogenesis via activation of the mitogen-activated protein kinase ERK-dependent pathway, is upregulated in neutrophils by IL-1beta, and functions as a novel inhibitor of apoptosis in response to a variety of inflammatory stimuli. It acts as a regulator of NAD+-dependent reactions in smooth muscle cells (SMCs) and in the pathogenesis of type 2 diabetes mellitus. NAMPT plays a role in insulin resistance and markers of hyperandrogenism in lean PCOS patients. It is a longevity protein that can add stress-resistant life to human smooth muscle cells by optimizing SIRT1-mediated p53 degradation. The expression level of NAMPT is high in visceral fat and serum levels in overweight and obese subjects with metabolic syndrome. It is expressed in large amounts in bone marrow, liver tissue, muscle, heart, placenta, lung and kidney tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAMPT
• Uniprot:
  • P43490()