Pax6 Antibody (1C8)

Western Blot: Pax6 Antibody (1C8) [NBP1-51622] - Western blot analysis using PAX6 moues mAb against recombinant PAX6 protein (1).

Flow Cytometry: Pax6 Antibody (1C8) [NBP1-51622] - Flow cytometric analysis of 3T3-L1 cells using PAX6 mouse mAb (green) and negative control (purple).

Product Discontinued

Pax6 Antibody (1C8) Summary

• Immunogen: Purified recombinant fragment of human PAX6 expressed in E. Coli.
• Marker: Neural Marker
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:2000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:34145313)
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Pax6 Antibody (1C8)

• keratitis)
• MGC17209
• Oculorhombin
• paired box 6
• paired box protein Pax-6
• Pax6

Background

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pax6 Antibody (NBP1-51622)(1)

Publication using NBP1-51622

• Doucet-O'Hare TT, DiSanza BL, DeMarino C et al. SMARCB1 deletion in atypical teratoid rhabdoid tumors results in human endogenous retrovirus K (HML-2) expression Scientific reports 2021-06-18 [PMID: 34145313] (ICC/IF, Human)

FAQs for Pax6 Antibody (NBP1-51622). (Showing 1 - 1 of 1 FAQ).

1. What PAX6 antibody is recommend to use in ICC staining of human stem cells differentiated neural precursors?
   • By performing a search on PAX6 then refining this to antibodies which have been validated with human samples and for ICC, I can see that we have three products to meet your requirements. You can see all three of these antibodies. These products are listed based on sales and citations, however all of our antibodies are covered by our 100% guarantee to work in the species and applications listed on our website and on our product datasheets. You can read more about our quality guarantee here.

Bioinformatics

• Gene Symbol: PAX6