Pax2 Antibody (PAX2/1104)

Immunohistochemistry-Paraffin: Pax2 Antibody (PAX2/1104) [NBP2-79742] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Pax2 Antibody (PAX2/1104).

Immunohistochemistry-Paraffin: Pax2 Antibody (PAX2/1104) [NBP2-79742] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Pax2 Antibody (PAX2/1104).

Immunohistochemistry-Paraffin: Pax2 Antibody (PAX2/1104) [NBP2-79742] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Pax2 Antibody (PAX2/1104).

Protein Array: Pax2 Antibody (PAX2/1104) [NBP2-79742] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Pax2 Antibody (PAX2/1104). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: ELISA, IHC, MA
• Clone: PAX2/1104
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Pax2 Antibody (PAX2/1104) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79877)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment (around aa 223-354) human Pax2 protein (exact sequence is proprietary) (Uniprot: Q02962)
• Localization: Nuclear
• Marker: Renal Cell & Ovarian Carcinoma Marker
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAX2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: ELISA: For coating, order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Pax2 Antibody (PAX2/1104)

• paired box 2
• paired box gene 2
• paired box homeotic gene 2
• paired box protein Pax-2
• Pax2

Background

PAX2 encodes paired box gene 2, one of many human homologues of the Drosophila melanogaster gene prd. The central feature of this transcription factor gene family is the conserved DNA-binding paired box domain. PAX2 is believed to be a target of transcriptional supression by the tumor supressor gene WT1. Mutations within PAX2 have been shown to result in optic nerve colobomas and renal hypoplasia. Alternative splicing of this gene results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAX2
• Uniprot:
  • Q02962()
  • Q02962()
  • Q02962()
  • Q02962()