PAWR / PAR4 Antibody (10Q2G9)

Western Blot: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Western blot analysis of extracts of various cell lines, using PAWR / PAR4 Rabbit mAb (NBP3-16523) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/Immunofluorescence: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Immunofluorescence analysis of HeLa cells using PAWR / PAR4 Rabbit mAb (NBP3-16523) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: PAWR / PAR4 Antibody (10Q2G9) [NBP3-16523] - Immunohistochemistry of paraffin-embedded human colon carcinoma using PAWR / PAR4 Rabbit mAb (NBP3-16523) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: 10Q2G9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PAWR / PAR4 Antibody (10Q2G9) Summary

• Description: Novus Biologicals Rabbit PAWR/ PAR4 Antibody (10Q2G9) (NBP3-16523) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 241-340 of human PAWR / PAR4 (Q96IZ0). YSRTDRSGFPRYNRDANVSGTLVSSSTLEKKIEDLEKEVVRERQENLRLVRLMQDKEEMIGKLKEEIDLLNRDLDDIEDENEQLKQENKTLLKVVGQLTR
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PAWR
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PAWR / PAR4 Antibody (10Q2G9)

• par-4
• PAR4prostate apoptosis response protein 4
• PRKC apoptosis WT1 regulator protein
• PRKC, apoptosis, WT1, regulator
• Prostate apoptosis response 4 protein
• prostate apoptosis response protein PAR-4
• transcriptional repressor PAR4
• WT1-interacting protein

Background

The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAWR