PASK Antibody (OTI8G9)

Western Blot: PASK Antibody (8G9) [NBP2-02242] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PASK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PASK.

Immunocytochemistry/Immunofluorescence: PASK Antibody (8G9) [NBP2-02242] Staining of COS7 cells transiently transfected by pCMV6-ENTRY PASK.

Immunohistochemistry-Paraffin: PASK Antibody (8G9) [NBP2-02242] - Staining of paraffin-embedded Human pancreas tissue using anti-PASK mouse monoclonal antibody.

Flow Cytometry: PASK Antibody (8G9) [NBP2-02242] - Analysis of Jurkat cells, using anti-PASK antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry-Paraffin: PASK Antibody (8G9) [NBP2-02242] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PASK mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PASK Antibody (8G9) [NBP2-02242] - Staining of paraffin-embedded Human Kidney tissue using anti-PASK mouse monoclonal antibody.

Immunohistochemistry-Paraffin: PASK Antibody (8G9) [NBP2-02242] - Staining of paraffin-embedded Human liver tissue using anti-PASK mouse monoclonal antibody.

Flow Cytometry: PASK Antibody (8G9) [NBP2-02242] - Analysis of Hela cells, using anti-PASK antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI8G9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.93 mg/ml

PASK Antibody (OTI8G9) Summary

• Immunogen: Full length human recombinant protein of human PASK (NP_055963) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PASK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
• Theoretical MW: 142.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.93 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PASK Antibody (OTI8G9)

• DKFZp434O051
• EC 2.7.11.1
• hPASK
• KIAA0135DKFZp686P2031
• PAS domain containing serine/threonine kinase
• PAS-kinase
• PASKINPAS domain-containing serine/threonine-protein kinase
• PAS-serine/threonine kinase
• STK37

Background

PAS domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. PASK is an evolutionarily conserved protein present in yeast, flies, and mammals.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PASK
• Entrez:
  • 23178(Human)