Park7/DJ-1 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Park7/DJ-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Park7/DJ-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Park7/DJ‑1 at approximately 28 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Park7 knockout HEK293T cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Park7/DJ-1 at approximately 23 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Park7/DJ‑1 was detected in immersion fixed paraffin-embedded sections of Human Brain Hypothalamus using Goat Anti-Human Park7/DJ‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Park7/DJ-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Park7/DJ-1
  Met1-Asp189
  Accession # Q99497
• Specificity: Detects human Park7/DJ-1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PARK7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Knockout Validated
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Park7/DJ-1 Antibody [Unconjugated]

• DJ1
• DJ-1
• DJ1FLJ34360
• EC 3.4
• FLJ27376
• FLJ92274
• Oncogene DJ1
• Park7
• Parkinson disease (autosomal recessive, early onset) 7
• Parkinson disease protein 7
• protein DJ-1

Background

Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson’s disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Park7/DJ-1 Antibody (AF3995)(3)

Publications using AF3995

• François Moreau, Aurélie Thédrez, Damien Garçon, Audrey Ayer, Thibaud Sotin, Wieneke Dijk et al. PCSK9 is not secreted from mature differentiated intestinal cells Journal of Lipid Research 2021-07-17 [PMID: 34280453]
• Niels Kruse, Michael G. Schlossmacher, Walter J. Schulz-Schaeffer, Eugeen Vanmechelen, Hugo Vanderstichele, Omar M. El-Agnaf et al. A First Tetraplex Assay for the Simultaneous Quantification of Total alpha -Synuclein, Tau, beta -Amyloid42 and DJ-1 in Human Cerebrospinal Fluid PLOS ONE 2016-04-26 [PMID: 27116005]
• Niels Kruse, Michael G. Schlossmacher, Walter J. Schulz-Schaeffer, Eugeen Vanmechelen, Hugo Vanderstichele, Omar M. El-Agnaf et al. A First Tetraplex Assay for the Simultaneous Quantification of Total alpha -Synuclein, Tau, beta -Amyloid42 and DJ-1 in Human Cerebrospinal Fluid PLOS ONE 2016-04-26 [PMID: 27116005] (ELISA Capture, ELISA Detection, Human)

Bioinformatics

• Gene Symbol: PARK7
• Uniprot:
  • Q99497()