Park7/DJ-1 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Park7/DJ-1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Park7/DJ-1
  Met1-Asp189
  Accession # Q99497
• Specificity: Detects human Park7/DJ-1 in Western blots. In this format, approximately 40% cross-reactivity with recombinant mouse Park7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PARK7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Park7/DJ-1 Antibody [Biotin]

• DJ1
• DJ-1
• DJ1FLJ34360
• EC 3.4
• FLJ27376
• FLJ92274
• Oncogene DJ1
• Park7
• Parkinson disease (autosomal recessive, early onset) 7
• Parkinson disease protein 7
• protein DJ-1

Background

Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson’s disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Park7/DJ-1 Antibody (BAF3995)(3)

Publications using BAF3995

• Wen-Yan Kang, Qiong Yang, Xu-Feng Jiang, Wei Chen, Lin-Yuan Zhang, Xiao-Ying Wang et al. Salivary DJ-1 could be an indicator of Parkinson's disease progression Frontiers in Aging Neuroscience 2014-06-06 [PMID: 24936184] (Luminex (Detection), Human)
• Wen-Yan Kang, Qiong Yang, Xu-Feng Jiang, Wei Chen, Lin-Yuan Zhang, Xiao-Ying Wang et al. Salivary DJ-1 could be an indicator of Parkinson's disease progression Frontiers in Aging Neuroscience 6/6/2014 [PMID: 24936184]
• Hong , Zhen, Shi , Min, Chung , Kathryn, Quinn , Joseph F, Peskind , Elaine R, Galasko , Douglas, Jankovic , Joseph, Zabetian , Cyrus P, Leverenz , James B, Baird , Geoffrey, Montine , Thomas J, Hancock , Aneeka M, Hwang , Hyejin, Pan , Catherin, Bradner , Joshua, Kang , Un J, Jensen , Poul H, Zhang , Jing DJ-1 and alpha-synuclein in human cerebrospinal fluid as biomarkers of Parkinson's disease. Brain, 2010-02-15;133(0):713-26. 2010-02-15 [PMID: 20157014] (Luminex Development, Human)

Bioinformatics

• Gene Symbol: PARK7
• Uniprot:
  • Q99497()