Park7/DJ-1 Antibody (S04-6H5)

Western Blot: Park7/DJ-1 Antibody (S04-6H5) [NBP3-20087] - Western blot detection of Park7/DJ-1 in K562, Rat Brain, C6, 3T3, Hela cell lysates using NBP3-20087 (1:1000 diluted). Predicted band size: 20kDa. Observed band size: 22kDa.

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody (S04-6H5) [NBP3-20087] - Immunohistochemistry of Park7/DJ-1 in paraffin-embedded Human tonsil using NBP3-20087 at dilution 1/100

Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody (S04-6H5) [NBP3-20087] - Immunofluorescence of Park7/DJ-1 (green) in Hela using NBP3-20087 at dilution 1/2, and DAPI (blue)

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S04-6H5
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

Park7/DJ-1 Antibody (S04-6H5) Summary

• Description: Novus Biologicals Rabbit Park7/DJ-1 Antibody (S04-6H5) (NBP3-20087) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Park7/DJ-1 (Uniprot # Q99497)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PARK7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20-1/100
  • Immunohistochemistry 1/50-1/200
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for Park7/DJ-1 Antibody (S04-6H5)

• DJ1
• DJ-1
• DJ1FLJ34360
• EC 3.4
• FLJ27376
• FLJ92274
• Oncogene DJ1
• Park7
• Parkinson disease (autosomal recessive, early onset) 7
• Parkinson disease protein 7
• protein DJ-1

Background

PARK7, also known as DJ-1, is a member of the peptidase C56 protein family. The PARK-7 protein is a positive regulator of androgen receptor-dependent transcription. It has also been reported to be a potential sensor for oxidative stress, a redox-sensitive chaperone, and a key protein for preventing neuronal oxidative stress and cell death.

PARK7 is highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart, and is detected at slightly lower levels in placenta and brain. The protein is also detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa.

Defects in this gene cause the autosomal recessive neurodegenerative disorder early-onset Parkinson's disease 7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PARK7