PARC Antibody - BSA Free

Western Blot: PARC Antibody [NB200-187] - Detection of Human PARC on HeLa whole cell lysate using NB200-187. PARC was also immunoprecipited using NB200-186. Immunoprecipitated PARC was blotted using NB200-187.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PARC Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PARC Antibody - BSA Free (NB200-187) is a polyclonal antibody validated for use in WB and IP. Anti-PARC Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide, mapping to a region between residue 2475 and the C-terminus (residue 2527) of human P53-associated parkin-like cytoplasmic protein using the numbering given in TrEMBL entry Q8IWT3 (GeneID 23113).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUL9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:20000-1:30000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PARC Antibody - BSA Free

• CUL-9
• cullin 9
• cullin-9
• DKFZp686G1042
• H7AP1DKFZp686P2024
• p53-associated parkin-like cytoplasmic protein
• PARCKIAA0708
• parkin-like cytoplasmic p53 binding protein
• RP3-330M21.2
• UbcH7-associated protein 1

Background

PARC is a cytoplasmic anchor protein in p53 associated protein complexes. PARC directly interacted and formed an approximately 1MD complex with p53 in the cytoplasm of unstressed cells. In the absence of stress, inactivation of PARC induced nuclear localization of endogenous p53 and activated p53 dependent apoptosis. Overexpression of PARC promoted cytoplasmic sequestration of ectopic p53. Furthermore, abnormal cytoplasmic localization of p53 was observed in a number of neuroblastoma cell lines; RNA interference-mediated reduction of endogenous PARC significantly sensitized these neuroblastoma cells in the DNA damage response. These results revealed that PARC is a critical regulator in controlling p53 subcellular localization and subsequent function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PARC Antibody (NB200-187)(1)

Publication using NB200-187

• Minamino M, Tei S, Negishi L, Kanemaki MT et Al. Temporal Regulation of ESCO2 Degradation by the MCM Complex, the CUL4-DDB1-VPRBP Complex, and the Anaphase-Promoting Complex Curr Biol 2018-08-14 [PMID: 30100344]

Bioinformatics

• Gene Symbol: CUL9
• Entrez:
  • 23113(Human)
  • 78309(Mouse)
• Uniprot:
  • Q8IWT3(Human)