Human CCL18/PARC DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human CCL18/PARC DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL18

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CCL18/PARC DuoSet ELISA, 15 Plate

• Alternative macrophage activation-associated CC chemokine 1
• AMAC-1
• AMAC1CC chemokine ligand 18
• AMAC-1Small-inducible cytokine A18
• CCL18
• chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
• CKb7
• DC-CK1
• DC-CK1CC chemokine PARC
• DCCK1Macrophage inflammatory protein 4
• Dendritic cell chemokine 1
• MIP4
• MIP-4
• MIP-4C-C motif chemokine 18
• PARC
• PARCPulmonary and activation-regulated chemokine
• SCYA18chemokine (C-C), dendritic
• small inducible cytokine A18
• small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

Background

Pulmonary and activation-regulated chemokine (PARC), also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid sequence identity). In vitro, PARC mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-gamma.

Publications for CCL18/PARC (DY394)(35)

Publications using DY394

• Parau, AC;Büyüksungur, S;Li, G;Liu, Q;Badillo, E;Blum, L;Schmidt, J;Pana, I;Vitelaru, C;Marinescu, IM;Dinu, M;Smuglov, M;Schmuttermaier, C;Tanir, TE;Klüter, H;Hasirci, N;Kzhyshkowska, J;Dragomir, AV; Zn-doped CaP coating equips Ti implants with corrosion resistance, biomineralization, antibacterial and immunotolerant activities Journal of advanced research 2025-01-27 [PMID: 39880075] (Human)
• Brollo, M;Salvator, H;Grassin-Delyle, S;Glorion, M;Descamps, D;Buenestado, A;Naline, E;Tenor, H;Tiotiu, A;Devillier, P; The IL-4/13-induced production of M2 chemokines by human lung macrophages is enhanced by adenosine and PGE2 International immunopharmacology 2024-01-23 [PMID: 38266451] (Human)
• Scherr, BF;Reiner, MF;Baumann, F;Höhne, K;Müller, T;Ayata, K;Müller-Quernheim, J;Idzko, M;Zissel, G; Prevention of M2 polarization and temporal limitation of differentiation in monocytes by extracellular ATP BMC immunology 2023-06-23 [PMID: 37353774] (Human)
• SI Johanns, RG Gantin, B Wangala, K Komlan, WA Halatoko, M Banla, P Karabou, AJ Luty, H Schulz-Key, C Köhler, PT Soboslay Onchocerca volvulus-specific antibody and cellular responses in onchocerciasis patients treated annually with ivermectin for 30 years and exposed to parasite transmission in central Togo PloS Neglected Tropical Diseases, 2022-05-03;16(5):e0010340. 2022-05-03 [PMID: 35503786] (Human)
• I Shefler, P Salamon, T Zitman-Gal, YA Mekori Tumor-Derived Extracellular Vesicles Induce CCL18 Production by Mast Cells: A Possible Link to Angiogenesis Cells, 2022-01-21;11(3):. 2022-01-21 [PMID: 35159163] (Human)
• B Grüner, L Peters, A Hillenbran, P Vo beta berg, J Schweiker, EG Rollmann, LH Rodriguez, J Blumhardt, S Burkert, P Kern, C Köhler, PT Soboslay Echinococcus multilocularis specific antibody, systemic cytokine, and chemokine levels, as well as antigen-specific cellular responses in patients with progressive, stable, and cured alveolar echinococcosis: A 10-year follow-up PloS Neglected Tropical Diseases, 2022-02-02;16(2):e0010099. 2022-02-02 [PMID: 35108275] (Human)
• T Dönmez, K Höhne, G Zissel, K Herrmann, H Hautzel, C Aigner, B Hegedüs, T Ploenes Insights into immunometabolism: A dataset correlating the 18FDG PET/CT maximum standard uptake value of the primary tumor with the CCL18 serum level in non-small cell lung cancer Data in Brief, 2021-02-18;35(0):106859. 2021-02-18 [PMID: 33665255] (Human)
• C Caliskan, B Seeliger, B Jäger, J Fuge, T Welte, O Terwolbeck, J Freise, CHM van Moorse, Y Zhang, A Prasse Genetic Variation in CCL18 Gene Influences CCL18 Expression and Correlates with Survival in Idiopathic Pulmonary Fibrosis-Part B J Clin Med, 2020-06-25;9(6):. 2020-06-25 [PMID: 32630441] (Human)
• F Zhang, EA Ayaub, B Wang, E Puchulu-Ca, YH Li, SU Hettiarach, SD Lindeman, Q Luo, S Rout, M Srinivasar, A Cox, K Tsoyi, C Nickerson-, IO Rosas, PS Low Reprogramming of profibrotic macrophages for treatment of bleomycin-induced pulmonary fibrosis EMBO Mol Med, 2020-06-29;0(0):e12034. 2020-06-29 [PMID: 32597014] (Human)
• TS Jayme, G Leung, A Wang, ML Workentine, S Rajeev, A Shute, BE Callejas, N Mancini, PL Beck, R Panaccione, DM McKay Human interleukin-4-treated regulatory macrophages promote epithelial wound healing and reduce colitis in a mouse model Sci Adv, 2020-06-05;6(23):eaba4376. 2020-06-05 [PMID: 32548267] (Human)
• YT Chang, HY Peng, CM Hu, SC Huang, SC Tien, YM Jeng Pancreatic cancer-derived small extracellular vesical Ezrin regulates macrophage polarization and promotes metastasis Am J Cancer Res, 2020-01-01;10(1):12-37. 2020-01-01 [PMID: 32064151] (Human)
• R Mills, A Mathur, LM Nicol, JJ Walker, AA Przybylski, AC Mackinnon, SEM Howie, WAH Wallace, I Dransfield, N Hirani Intrapulmonary Autoantibodies to HSP72 Are Associated with Improved Outcomes in IPF J Immunol Res, 2019-04-11;2019(0):1845128. 2019-04-11 [PMID: 31098385] (Human)
• M Ossorio, V Martínez, MA Bajo, G Del Peso, MJ Castro, S Romero, R Selgas, T Bellón Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists Biomed Res Int, 2018-04-04;2018(0):6415892. 2018-04-04 [PMID: 29850544] (Human)
• A Lescoat, A Ballerie, Y Augagneur, C Morzadec, L Vernhet, O Fardel, P Jégo, S Jouneau, V Lecureur Distinct Properties of Human M-CSF and GM-CSF Monocyte-Derived Macrophages to Simulate Pathological Lung Conditions In Vitro: Application to Systemic and Inflammatory Disorders with Pulmonary Involvement Int J Mol Sci, 2018-03-17;19(3):. 2018-03-17 [PMID: 29562615] (Human)
• E Gkika, W Vach, S Adebahr, T Schimeck-J, A Brenner, TB Brunner, K Kaier, A Prasse, J Müller-Que, AL Grosu, G Zissel, U Nestle Is serum level of CC chemokine ligand 18 a biomarker for the prediction of radiation induced lung toxicity (RILT)? PLoS ONE, 2017-09-28;12(9):e0185350. 2017-09-28 [PMID: 28957436] (Human)
• HM Rostam, PM Reynolds, MR Alexander, N Gadegaard, AM Ghaemmagha Image based Machine Learning for identification of macrophage subsets Sci Rep, 2017-06-14;7(1):3521. 2017-06-14 [PMID: 28615717] (Human)
• M Ossorio, MA Bajo, G Del Peso, V Martínez, M Fernández, MJ Castro, A Rodríguez-, R Madero, T Bellón, R Selgas Sustained low peritoneal effluent CCL18 levels are associated with preservation of peritoneal membrane function in peritoneal dialysis PLoS ONE, 2017-04-17;12(4):e0175835. 2017-04-17 [PMID: 28414753] (Human)
• Vagishwari Murugesan Validating glycoprotein non-metastatic melanoma B (gpNMB, osteoactivin), a new biomarker of Gaucher disease Blood Cells Mol. Dis, 2016-12-13;0(0):. 2016-12-13 [PMID: 28003098] (Human)
• Jaguin M, Fardel O, Lecureur V Exposure to diesel exhaust particle extracts (DEPe) impairs some polarization markers and functions of human macrophages through activation of AhR and Nrf2. PLoS ONE, 2015-02-24;10(2):e0116560. 2015-02-24 [PMID: 25710172] (Human)
• Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis. PLoS ONE, 2015-01-15;10(1):e0116775. 2015-01-15 [PMID: 25590613] (Human)
• Maddur M, Sharma M, Hegde P, Stephen-Victor E, Pulendran B, Kaveri S, Bayry J Human B cells induce dendritic cell maturation and favour Th2 polarization by inducing OX-40 ligand. Nat Commun, 2014-06-09;5(0):4092. 2014-06-09 [PMID: 24910129] (Human)
• L van Bon, AJ Affandi, J Broen, RB Christmann, RJ Marijnisse, L Stawski, GA Farina, G Stifano, AL Mathes, M Cossu, M York, C Collins, M Wenink, R Huijbens, R Hesselstra, T Saxne, M DiMarzio, D Wuttge, SK Agarwal, JD Reveille, S Assassi, M Mayes, Y Deng, JP Drenth, J de Graaf, M den Heijer, CG Kallenberg, M Bijl, A Loof, WB van den Be, LA Joosten, V Smith, F de Keyser, R Scorza, C Lunardi, PL van Riel, M Vonk, W van Heerde, S Meller, B Homey, L Beretta, M Roest, M Trojanowsk, R Lafyatis, TR Radstake Proteome-wide analysis and CXCL4 as a biomarker in systemic sclerosis N. Engl. J. Med, 2013-12-18;370(5):433-43. 2013-12-18 [PMID: 24350901] (Human)
• Stahl M, Schupp J, Jager B, Schmid M, Zissel G, Muller-Quernheim J, Prasse A Lung collagens perpetuate pulmonary fibrosis via CD204 and M2 macrophage activation. PLoS ONE, 2013-11-20;8(11):e81382. 2013-11-20 [PMID: 24278429] (Human)
• Plones T, Krohn A, Burger M, Veelken H, Passlick B, Muller-Quernheim J, Zissel G Serum level of CC-chemokine ligand 18 is increased in patients with non-small-cell lung cancer and correlates with survival time in adenocarcinomas. PLoS ONE, 2012-07-25;7(7):e41746. 2012-07-25 [PMID: 22848587] (Human)
• Pavlova EV, Deegan PB, Tindall J, McFarlane I, Mehta A, Hughes D, Wraith JE, Cox TM Potential biomarkers of osteonecrosis in Gaucher disease. Blood Cells Mol. Dis., 2010-11-13;46(1):27-33. 2010-11-13 [PMID: 21075651] (Human)
• Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int. J. Cancer, 2009-07-15;125(2):367-73. 2009-07-15 [PMID: 19378341] (Human)
• Li X, Syrovets T, Paskas S, Laumonnier Y, Simmet T Mature dendritic cells express functional thrombin receptors triggering chemotaxis and CCL18/pulmonary and activation-regulated chemokine induction. J. Immunol., 2008-07-15;181(2):1215-23. 2008-07-15 [PMID: 18606675] (Human)
• Lemarie A, Bourdonnay E, Morzadec C, Fardel O, Vernhet L Inorganic arsenic activates reduced NADPH oxidase in human primary macrophages through a Rho kinase/p38 kinase pathway. J. Immunol., 2008-05-01;180(9):6010-7. 2008-05-01 [PMID: 18424721] (Human)
• Duluc D, Delneste Y, Tan F, Moles MP, Grimaud L, Lenoir J, Preisser L, Anegon I, Catala L, Ifrah N, Descamps P, Gamelin E, Gascan H, Hebbar M, Jeannin P Tumor-associated leukemia inhibitory factor and IL-6 skew monocyte differentiation into tumor-associated macrophage-like cells. Blood, 2007-09-11;110(13):4319-30. 2007-09-11 [PMID: 17848619] (Human)
• Prasse A, Pechkovsky DV, Toews GB, Schafer M, Eggeling S, Ludwig C, Germann M, Kollert F, Zissel G, Muller-Quernheim J CCL18 as an indicator of pulmonary fibrotic activity in idiopathic interstitial pneumonias and systemic sclerosis. Arthritis Rheum., 2007-05-01;56(5):1685-93. 2007-05-01 [PMID: 17469163] (Human)
• Momohara S, Okamoto H, Iwamoto T, Mizumura T, Ikari K, Kawaguchi Y, Takeuchi M, Kamatani N, Tomatsu T High CCL18/PARC expression in articular cartilage and synovial tissue of patients with rheumatoid arthritis. J. Rheumatol., 2007-02-01;34(2):266-71. 2007-02-01 [PMID: 17304652] (Human)
• Chang Y, Senechal S, de Nadai P, Chenivesse C, Gilet J, Vorng H, Legendre B, Tonnel AB, Wallaert B, Lassalle P, Tsicopoulos A Diesel exhaust exposure favors TH2 cell recruitment in nonatopic subjects by differentially regulating chemokine production. J. Allergy Clin. Immunol., 2006-06-21;118(2):354-60. 2006-06-21 [PMID: 16890758] (Human)
• Luzina IG, Papadimitriou JC, Anderson R, Pochetuhen K, Atamas SP Induction of prolonged infiltration of T lymphocytes and transient T lymphocyte-dependent collagen deposition in mouse lungs following adenoviral gene transfer of CCL18. Arthritis Rheum., 2006-08-01;54(8):2643-55. 2006-08-01 [PMID: 16868995] (Human)
• Gunther C, Bello-Fernandez C, Kopp T, Kund J, Carballido-Perrig N, Hinteregger S, Fassl S, Schwarzler C, Lametschwandtner G, Stingl G, Biedermann T, Carballido JM CCL18 is expressed in atopic dermatitis and mediates skin homing of human memory T cells. J. Immunol., 2005-02-01;174(3):1723-8. 2005-02-01 [PMID: 15661937] (Human)
• Pivarcsi A, Gombert M, Dieu-Nosjean MC, Kubitza R, Meller S, Rieker J, Muller A, Da Cunha L, Haahtela A, Sonkoly E, Fridman WH, Alenius H, Kemeny L, Ruzicka T, Zlotnik A CC chemokine ligand 18, an atopic dermatitis-associated and dendritic cell-derived chemokine, is regulated by staphylococcal products and allergen exposure. J. Immunol., 2004-11-01;173(9):5810-7. 2004-11-01 [PMID: 15494534] (Human)

FAQs for CCL18/PARC (DY394). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL18