Pappalysin-1/PAPP-A Antibody (3C8)

Images

 
Western Blot: PAPP A Antibody (3C8) [NB200-403] - Figure A - SDS-PAGE analysis in reducing conditions of human recombinant dPAPP-A and tissue (purified from atherosclerotic plaques) dPAPP-A. Lane 1: human htPAPP-A ...read more
Western Blot: PAPP A Antibody (3C8) [NB200-403] - Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blotting. Lane 1: 18A10 (NB100-73059) Lane 2: 7A6 (NB100-73060) Lane 3: ...read more
Sandwich ELISA: PAPP A Antibody (3C8) [NB200-403] - Calibration curves for PAPP-A sanwich immunoassay. (A) 4G11 3C8 Capture Mab: 4G11 (biotinylated) Detection MAbs: 3C8 (labeled with stable Eu3+ -chelate) Antigen: ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP, S-ELISA
Clone
3C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Pappalysin-1/PAPP-A Antibody (3C8) Summary

Immunogen
Purified human PAPPA antigen.
Localization
Secreted
Specificity
PAPPA (3C8)
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PAPPA
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
Application Notes
This antibody works well in htPAPP-A and dPAPP-A (PAPP-A dimer) immunoassays, Immunoprecipitation and PAPP-A immunodetection in Western blotting. Recommended pair for the detection of dPAPP-A antigen in blood of AMI patients and patients with unstable angina (capture - detection): NB200-401 (Capture) - NB200-403 (Detection). Best pair for the detection of htPAPP-A antigen in pregnancy serum (capture - detection): NB200-401(Capture) - NB200-403 (Detection)

Reactivity Notes

Cross-reacts with Human. Not yet tested in other species.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Purity
Protein A purified

Alternate Names for Pappalysin-1/PAPP-A Antibody (3C8)

  • ASBABP2
  • DIPLA1
  • EC 3.4.24.79
  • IGFBP-4ase
  • IGFBP-4aseaspecific BCL2 ARE-binding protein 2
  • IGF-dependent IGFBP-4 protease
  • insulin-like growth factor-dependent IGF binding protein-4 protease
  • Insulin-like growth factor-dependent IGF-binding protein 4 protease
  • PAPA
  • PAPPA
  • PAPPA1
  • PAPP-A1
  • PAPP-Adifferentially placenta 1 expressed protein
  • Pappalysin1
  • Pappalysin-1
  • pregnacy-associated plasma protein A
  • Pregnancy-associated plasma protein A
  • pregnancy-associated plasma protein A, pappalysin 1

Background

PAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Pappalysin-1/PAPP-A Antibody (NB200-403). (Showing 1 - 1 of 1 FAQ).

  1. I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).
    • Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAPPA
Entrez