Pappalysin-1/PAPP-A Antibody (10E1cc) - BSA Free

Western Blot: PAPP A Antibody (10E1) [NB120-8346] - Detection of human PAPP-A and proMBP subunits of htPAPP-A by monoclonal antibodies in Western blotting. Lane 1: 18A10 (NB100-73059) Lane 2: 7A6 (NB100-73060) Lane 3: 3C8 (NB200-402) Lane 4: 5H9 (proMBP-specific) (NB110-8423) Lane 5: 11E4 (proMBP-specific) (NB120-14597) Lane 6: 7A6 (NB100-73060) Lane 7: 10A5 (NB200-402) Lane 8: 3C8 (NB200-403) Lane 9: 10E1 (NB120-8346) Lanes 1-5: after SDS-PAGE in reduction conditions. Lanes 6-9: Non-reducing conditions. Heterotetrameric complex was detected by anti-PAPP-A MAbs

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clone: 10E1cc
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Pappalysin-1/PAPP-A Antibody (10E1cc) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified human PAPP-A/proMBP (heterotetrameric PAPP-A, htPAPP-A) complex.
• Specificity: Human PAPP-A antigen purified from placental blood (heterotetrameric complex, consisting of PAPP-A subunits and proMBP subunits).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PAPPA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: htPAPP-A and dPAPP-A (PAPP-A dimer) immunoassays and immunoprecipitation. Recommended pairs for detection of total PAPP-A (htPAPP-A and/or dPAPP-A) (capture -detection): NB100-73058 - NB120-8346, NB120-8346 - NB100-73060.
  
  Recognizes htPAPP-A, consisting of PAPP-A subunits and proMBP subunits, in non-reducing conditions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Pappalysin-1/PAPP-A Antibody (10E1cc) - BSA Free

• ASBABP2
• DIPLA1
• EC 3.4.24.79
• IGFBP-4ase
• IGFBP-4aseaspecific BCL2 ARE-binding protein 2
• IGF-dependent IGFBP-4 protease
• insulin-like growth factor-dependent IGF binding protein-4 protease
• Insulin-like growth factor-dependent IGF-binding protein 4 protease
• PAPA
• PAPPA
• PAPPA1
• PAPP-A1
• PAPP-Adifferentially placenta 1 expressed protein
• Pappalysin1
• Pappalysin-1
• pregnacy-associated plasma protein A
• Pregnancy-associated plasma protein A
• pregnancy-associated plasma protein A, pappalysin 1

Background

PAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Pappalysin-1/PAPP-A Antibody (NB120-8346) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/01/2021

Summary

• Application: Western Blot
• Sample Tested: Human serum
• Species: Human
• Lot: 10E1cc

FAQs for Pappalysin-1/PAPP-A Antibody (NB120-8346). (Showing 1 - 1 of 1 FAQ).

1. I am highly interested to use couple of Anti-PAPP-A antibodies from your reputed companies for my research work. I have got the information regarding the immunogen used to generate these antibodies, however could not get any information regarding the specific amino acids/epitope to which your Anti-PAPP-A Antibodies binds with the antigen (PAPP-A).
   • Unfortunately, none of our PAPP A antibodies have been epitope mapped. Many of them have also been generated against full-length PAPP A, so there are many potential binding sites.

Bioinformatics

• Gene Symbol: PAPPA
• Entrez:
  • 5069(Human)