PAPOLG Antibody - BSA Free

Western Blot: PAPOLG Antibody [NBP1-30061] - Carrier Free [NBP1-30061] - Samples: Whole cell lysate from HeLa and mouse NIH3T3 cells. PAP-gamma was also IPed by NBP1-30060. For blotting IPed PAP-gamma, the ReliaBLOT Reagents and Procedures were used.

• Reactivity: Hu, Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PAPOLG Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PAPOLG Antibody - BSA Free (NBP1-30061) is a polyclonal antibody validated for use in WB and IP. Anti-PAPOLG Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: maps to a region between residue 686 and 736 of human poly(A) polymerase gamma using the numbering given in entry NP_075045.2
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAPOLG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 10-15 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Theoretical MW: 83 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PAPOLG Antibody - BSA Free

• EC 2.7.7.19
• FLJ11805
• FLJ12972
• FLJ13482
• FLJ14187
• MGC133307
• MGC133308
• neo-PAP
• Neo-poly(A) polymerase
• nuclear poly(A) polymerase gamma
• PAP2
• PAPG
• PAP-gamma
• poly(A) polymerase gamma
• Polynucleotide adenylyltransferase gamma
• SRP RNA 3' adenylating enzyme/pap2
• SRP RNA 3'-adenylating enzyme

Background

PAPOLG is a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAPOLG Antibody (NBP1-30061)(1)

Publication using NBP1-30061

• Bresson SM, Conrad NK. The Human nuclear poly(a)-binding protein promotes RNA hyperadenylation and decay. PLoS Genet. 2013-10-01 [PMID: 24146636] (WB, Human)

Bioinformatics

• Gene Symbol: PAPOLG
• Entrez:
  • 64895(Human)
• Uniprot:
  • Q9BWT3(Human)