pan Cadherin Antibody

Western Blot: pan Cadherin Antibody [H00000999-D01P] - Analysis of CDH1 expression in human pancreas.

Western Blot: pan Cadherin Antibody [H00000999-D01P] - Analysis of CDH1 expression in mouse lung.

Product Discontinued

pan Cadherin Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian tissue lysate.
• Immunogen: CDH1 (ENSP00000261769, 1 a.a. - 882 a.a.) full-length human protein. MGPWSRSLSALLLLLQVSSWLCQEPEPCHPGFDAESYTFTVPRRHLERGRVLGRVNFEDCTGRQRTAYFSLDTRFKVGTDGVITVKRPLRFHNPQIHFLVYAWDSTYRKFSTKVTLNTVGHHHRPPPHQASVSGIQAELLTFPNSSPGLRRQKRDWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIPAILGILGGILALLILILLLLLFLRRRAVVKEPLLPPEDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPRYLPRPANPDEIGNFIDENLKAADTDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESDKDQDYDYLNEWGNRFKKLADMYGGGEDD
• Marker: Plasma Membrane Marker
• Specificity: Reacts with cadherin 1, type 1, E-cadherin (epithelial).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Functional
  • Western Blot
• Application Notes: This antibody is reactive against transfected lysate and as a detection antibody in ELISA.

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 24040379).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for pan Cadherin Antibody

• Arc-1
• Cadherin (pan)
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDHE
• cell-CAM 120/80
• ECAD
• E-cadherin
• Epithelial cadherin
• LCAM
• UVOE-Cadherin
• Uvomorulin

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for pan Cadherin Antibody (H00000999-D01P)(3)

Publications using H00000999-D01P

• Yang H, Zhang Y, Zhou Z et al. Transcription factor Snai1-1 induces osteosarcoma invasion and metastasis by inhibiting E-cadherin expression. Oncol Lett. 2014-04-22 [PMID: 24959244]
• Hall MK, Weidner DA, Chen JM et al. Glycan structures contain information for the spatial arrangement of glycoproteins in the plasma membrane. PLoS One. 2013-09-06 [PMID: 24040379] (WB, Hamster)
• Wen CL, Chen KY, Chen CT et al. Development of an AlphaLISA assay to quantify serum core-fucosylated E-cadherin as a metastatic lung adenocarcinoma biomarker. J Proteomics 2012-05-23 [PMID: 22634079]

Review for pan Cadherin Antibody (H00000999-D01P) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer WB Other 03/28/2014

Summary

• Application: Western Blot
• Species: Other

FAQs for pan Cadherin Antibody (H00000999-D01P). (Showing 1 - 1 of 1 FAQs).

1. What concentration of anti-pan Cadherin Ab was used to for the Western blots shown above?
   • This product is an Abnova product and they typically dilute the Ab with a dilution range of 1:500-1:1000.

Bioinformatics

• Gene Symbol: CDH1
• Entrez:
  • 999(Human)
• Uniprot:
  • ENSP00000261769(Human)