PAK4 Antibody

Images

 
Western Blot: PAK4 Antibody [NBP1-77236] - SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 u/mL.
Immunohistochemistry-Paraffin: PAK4 Antibody [NBP1-77236] - Human colon tissue with PAK4 antibody at 10 ug/ml.
Immunofluorescence: PAK4 Antibody [NBP1-77236] - Human Colon tissue with PAK4 antibody at 20 ug/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

PAK4 Antibody Summary

Immunogen
Antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4. The immunogen is located within amino acids 210 - 260 of PAK4.
Specificity
PAK4 antibody is predicted to not cross-react with other PAK family proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAK4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence
Application Notes
This antibody can be used for the detection of PAK4 by Western blot at 1 - 2 mg/ml.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
PAK4 Peptide (NBP1-77236PEP)
Publications
Read Publication using
NBP1-77236 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PAK4 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • KIAA1142
  • p21 protein (Cdc42/Rac)-activated kinase 4
  • p21(CDKN1A)-activated kinase 4
  • p21-activated kinase 4
  • PAK4
  • PAK-4
  • protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
  • serine/threonine-protein kinase PAK 4

Background

The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PAK4 Antibody (NBP1-77236)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Filter By Species
Human
(1)
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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PAK4
Entrez
Uniprot