PAK4 Antibody - BSA Free

Western Blot: PAK4 Antibody [NBP1-77236] - SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 u/mL.

Immunohistochemistry: PAK4 Antibody - BSA Free [NBP1-77236] - Immunohistochemistry of PAK4 in human colon tissue with PAK4 antibody at 10 u/mL.

Immunocytochemistry/ Immunofluorescence: PAK4 Antibody - BSA Free [NBP1-77236] - Immunofluorescence of PAK4 in Human Colon tissue with PAK4 antibody at 20 ug/mL.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PAK4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PAK4 Antibody - BSA Free (NBP1-77236) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-PAK4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4. The immunogen is located within amino acids 210 - 260 of PAK4. Amino Acid Squence: GPSAGGLAIPQS
• Specificity: PAK4 antibody is predicted to not cross-react with other PAK family proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAK4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  PAK4 Peptide (NBP1-77236PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for PAK4 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• KIAA1142
• p21 protein (Cdc42/Rac)-activated kinase 4
• p21(CDKN1A)-activated kinase 4
• p21-activated kinase 4
• PAK4
• PAK-4
• protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs
• serine/threonine-protein kinase PAK 4

Background

The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6. These proteins are not activated by Cdc42/Rac binding. PAK4 was initially identified as a novel effector of Cdc42Hs. Co-expression of PAK4 and Cdc42Hs resulted in induction of filopodia and actin polymerization, showing that it is involved in cytoskeletal reorganization. Other experiments have shown PAK4 to be essential for embryonic viability and proper neuronal development. PAK4 has also been implicated in anchorage-independent growth of tumor cells and is required for activation of several cancer prosurvival pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PAK4 Antibody (NBP1-77236)(1)

Publication using NBP1-77236

• Liu W, Yang Y, Liu Y et al. p21-Activated kinase 4 predicts early recurrence and poor survival in patients with nonmetastatic clear cell renal cell carcinoma Urol. Oncol. 2015-03-02 [PMID: 25744653] (IHC-P, Human)

Bioinformatics

• Gene Symbol: PAK4
• Uniprot:
  • NP_005875(Human)