PAK2 Antibody - BSA Free

Images

 
Western Blot: PAK2 Antibody [NBP1-76720] - Western Blot Validation in Mouse Tissues. Loading: 15 ug of lysates per lane. Antibodies: PAK2 NBP1-76720 (1 ug/mL per lane), 1h incubation at RT in 5% NFDM/TBST. Secondary: ...read more
Immunocytochemistry/ Immunofluorescence: PAK2 Antibody [NBP1-76720] - Immunofluorescence of PAK2 in Mouse Spleen cells with PAK2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: PAK2 Antibody [NBP1-76720] - Mouse spleen tissue with PAK2 antibody at 10 ug/ml.
Western Blot: PAK2 Antibody [NBP1-76720] - Analysis of PAK2 in rat bladder tissue lysate with PAK2 antibody at (A) 0.5 and (B) 1 ug/mL.
Independent Antibodies: Western Blot: PAK2 Antibody [NBP1-76720] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: PAK2 ...read more
Western Blot: PAK2 Antibody [NBP1-76720] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: PAK2 NBP1-76720 (1 ug/mL per lane), 1h incubation at RT in 5% ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

PAK2 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human PAK2. The immunogen is located within amino acids 440 - 490 of PAK2. Amino Acid Squence: IATNGTPELQNPEK
Predicted Species
Rabbit (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PAK2
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-1 ug/ml
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
PAK2 Peptide (NBP1-76720PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for PAK2 Antibody - BSA Free

  • EC 2.7.11
  • gamma-PAK
  • p21 (CDKN1A)-activated kinase 2
  • p21 protein (Cdc42/Rac)-activated kinase 2
  • p21-activated kinase 2
  • p58
  • PAK2
  • PAK-2
  • PAK65
  • PAK65EC 2.7.11.1
  • PAKgamma
  • S6/H4 Kinase
  • serine/threonine-protein kinase PAK 2

Background

The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PAK2
Uniprot