LIM Kinase 1 Antibody (OTI3H6)

Images

 
Western Blot: LIM Kinase 1 Antibody (3H6) [NBP2-00748] Analysis of extracts (35ug) from 9 different cell lines by using anti-LIM Kinase 1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LIM Kinase 1.
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIM Kinase 1 mouse monoclonal antibody.
Flow Cytometry: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIM Kinase 1 antibody, and ...read more
Western Blot: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIM Kinase 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of ...read more
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (3H6) [NBP2-00748] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIM Kinase 1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3H6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

LIM Kinase 1 Antibody (OTI3H6) Summary

Immunogen
Full length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
LIMK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
72.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-00748.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LIM Kinase 1 Antibody (OTI3H6)

  • EC 2.7.11.1
  • LIM domain kinase 1
  • LIM Kinase 1
  • LIM motif-containing protein kinase
  • LIMK
  • LIMK1
  • LIMK-1
  • LIMKLIMK-1

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIM Kinase 1 Antibody (NBP2-00748)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIMK1
Entrez