PAK1 [p Thr84] Antibody - Azide Free

Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511] - Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ch, Gp, Pm, Sh, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

PAK1 [p Thr84] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit PAK1 [p Thr84] Antibody - Azide Free (NBP2-29511) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 conjugated to KLH. Accession # Q13153
• Modification: p Thr84
• Specificity: Specific for endogenous levels of the ~68 kDa PAK1 phosphorylated at Thr84. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
• Predicted Species: Primate (100%), Sheep (100%), Chicken (100%), Canine (100%), Bovine (100%), Guinea Pig (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAK1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for PAK1 [p Thr84] Antibody - Azide Free

• alpha-PAK
• EC 2.7.11
• EC 2.7.11.1
• MGC130000
• MGC130001
• p21 protein (Cdc42/Rac)-activated kinase 1
• p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
• p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
• p21-activated kinase 1
• p65-PAK
• PAK1
• PAK-1
• PAKalpha
• serine/threonine-protein kinase PAK 1
• STE20 homolog, yeast

Background

PAKs, p21 activated kinases, are a family of serine/threonine protein kinases comprised of six isoforms, PAK1-6, and they play important roles in cytoskeleton dynamics, cell survival and proliferation (Ye et al, 2012). Each of these isoforms contains a C-terminal catalytic domain and an N-terminal regulatory domain with a small G protein binding motif (Chen et al, 2004). OSR1, oxidative stress response 1, is activated only by osmotic stresses, like sorbital or NaCl (Chen et al, 2004). It has been predicted that OSR1 phosphorylates PAK1 in the regulatory domain at thr84 and inhibits activation of JNK and MAPK pathway. (Chen et al, 2004). It has also been suggested that OSR1 may have a regulating function with actin cytoskeleton because it can phosphorylate PAK1 at thr84 and bind to gelsolin (Chen et al, 2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAK1
• Entrez:
  • 5058(Human)
• Uniprot:
  • Q13153()