PAK1 Antibodies
We offer PAK1 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each PAK1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PAK1 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the PAK1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PAK1 polyclonal antibodies and browse our PAK1 monoclonal antibody catalog.
Alternate Names for PAK1 Antibodies
anti-PAK1 antibody, anti-alpha-PAK antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.1 antibody, anti-MGC130000 antibody, anti-MGC130001 antibody, anti-p21 protein (Cdc42/Rac)-activated kinase 1 antibody, anti-p21-activated kinase 1 antibody, anti-p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody, anti-p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related) antibody, anti-p65-PAK antibody, anti-PAK-1 antibody, anti-PAKalpha antibody, anti-serine/threonine-protein kinase PAK 1 antibody, anti-STE20 homolog, yeast antibody
16 Results for "pak1" in Primary Antibodies
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="PAK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human PAK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7495) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
![Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Western-Blot-H00005058-M02-img0011.jpg "Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on HeLa cell. Antibody concentration 35 ug/ml.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunocytochemistry-Immunofluorescence-H00005058-M02-img0006.jpg)
![Immunohistochemistry-Paraffin: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunohistochemistry-Paraffin-H00005058-M02-img0008.jpg)
![Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Western Blot analysis of PAK1 expression in HeLa NE ( Cat # L013V3 ).](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Western-Blot-H00005058-M02-img0001.jpg)
![Immunoprecipitation: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Immunoprecipitation-H00005058-M02-img0009.jpg)
![Sandwich ELISA: PAK1 Antibody (4D1) [H00005058-M02] - Detection limit for recombinant GST tagged PAK1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PAK1-Antibody-4D1-Sandwich-ELISA-H00005058-M02-img0010.jpg)
![Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680] - Paraffin-embedded mouse fore brain. PAK1 antibody [N2C2], Internal dilution: 1:500.](http://images.novusbio.com/fullsize/PAK1-Antibody-Immunohistochemistry-Paraffin-NBP2-19680-img0006.jpg "Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680] - Paraffin-embedded mouse fore brain. PAK1 antibody [N2C2], Internal dilution: 1:500.")
![Western Blot: PAK1 Antibody [NBP2-19680] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/PAK1-Antibody-Western-Blot-NBP2-19680-img0001.jpg)
![Western Blot: PAK1 Antibody [NBP2-19680] - Sample (30 ug of whole cell lysate) A: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/PAK1-Antibody-Western-Blot-NBP2-19680-img0002.jpg)
![Immunohistochemistry-Paraffin: PAK1 Antibody [NBP2-19680] - HBL435 xenograft.](http://images.novusbio.com/fullsize/PAK1-Antibody-Immunohistochemistry-Paraffin-NBP2-19680-img0004.jpg)
![PAK1 antibody [N2C2], Internal detects PAK1 protein by western blot analysis.<br />A. 30 ug PC-12 whole cell lysate/extract<br />7.5% SDS-PAGE<br />PAK1 antibody [N2C2], Internal (NBP2-19680) dilution: 1:1000 <br />The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19680_rabbit-polyclonal-pak1-antibody-western-blot-261120251819368.jpg)
![Western Blot: PAK1 Antibody [NBP3-04201] - Analysis of extracts of mouse kidney, using PAK1 antibody at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/PAK1-Antibody-Western-Blot-NBP3-04201-img0003.jpg "Western Blot: PAK1 Antibody [NBP3-04201] - Analysis of extracts of mouse kidney, using PAK1 antibody at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: PAK1 Antibody [NBP3-04201] - Paraffin-embedded human lung cancer using PAK1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PAK1-Antibody-Immunohistochemistry-Paraffin-NBP3-04201-img0002.jpg)
![Immunohistochemistry-Paraffin: PAK1 Antibody [NBP3-04201] - Paraffin-embedded rat spleen using PAK1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/PAK1-Antibody-Immunohistochemistry-Paraffin-NBP3-04201-img0001.jpg)
![Western Blot: PAK1 Antibody (SR2113) [NBP3-21849] - Western blot analysis using NBP3-21849 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21849_rabbit-pak1-mab-sr2113-57202316582145.jpg "Western Blot: PAK1 Antibody (SR2113) [NBP3-21849] - Western blot analysis using NBP3-21849 in A431 cell lysate.")
![Western Blot: PAK1 Antibody (6R3M5) [NBP3-15811] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP3-15811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PAK1-Antibody-6R3M5-Western-Blot-NBP3-15811-img0002.jpg "Western Blot: PAK1 Antibody (6R3M5) [NBP3-15811] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP3-15811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: PAK1 Antibody (6R3M5) [NBP3-15811] - Immunofluorescence analysis of NIH-3T3 cells using PAK1 antibody (NBP3-15811). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PAK1-Antibody-6R3M5-Immunocytochemistry-Immunofluorescence-NBP3-15811-img0001.jpg)
![Western Blot: PAK1 Antibody (4F10) [NBP3-15141] - Positive WB detected in: MCF7 whole cell lysate, JK whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, ntera2 whole cell lysate, Mouse Heart tissue lysate, Mouse Skeletal Muscle tissue lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate<br />All lanes: PAK1 antibody at 1:1000<br />Secondary<br />Goat polyclonal to rabbit IgG at 1/50000 dilution<br />Predicted band size: 61 kDa<br />Observed band size: 61 kDa<br />](http://images.novusbio.com/fullsize/nbp3-15141_rabbit-pak1-mab-4f10-26120251011410.jpg "Western Blot: PAK1 Antibody (4F10) [NBP3-15141] - Positive WB detected in: MCF7 whole cell lysate, JK whole cell lysate, K562 whole cell lysate, A549 whole cell lysate, ntera2 whole cell lysate, Mouse Heart tissue lysate, Mouse Skeletal Muscle tissue lysate, Mouse Brain tissue lysate, Rat Brain tissue lysate<br />All lanes: PAK1 antibody at 1:1000<br />Secondary<br />Goat polyclonal to rabbit IgG at 1/50000 dilution<br />Predicted band size: 61 kDa<br />Observed band size: 61 kDa<br />")
![Immunocytochemistry/Immunofluorescence: PAK1 Antibody (4F10) [NBP3-15141] - Staining of Hela cell with NBP3-15141 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-15141_rabbit-pak1-mab-4f10-2612025101146.jpg)
![Western Blot: PAK1 Antibody [NB100-41389] - (1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PAK1-Antibody-Western-Blot-NB100-41389-img0001.jpg "Western Blot: PAK1 Antibody [NB100-41389] - (1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PAK1 Antibody [NBP2-95107] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP2-95107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PAK1 Antibody-Western Blot-NBP2-95107-img0001.jpg "Western Blot: PAK1 Antibody [NBP2-95107] - Western blot analysis of extracts of various cell lines, using PAK1 antibody (NBP2-95107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: PAK1 Antibody - Azide and BSA Free [PAK1] - Western blot analysis of various lysates using PAK1 Rabbit pAb at 1:1000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit .<br>Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp2-95107_rabbit-polyclonal-pak1-antibody-5220251638053.jpg)
![Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511] - Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/PAK1-[p-Thr84]-Antibody-Western-Blot-NBP2-29511-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511] - Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665] - Untreated and pervanadate-treated NIH/3T3 cells (lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, phosphorylated-peptide blocked, or non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Lanes 4 and 5 were blocking peptides were the phosphorylated peptide used as the immunogen and the non-phopshrylated form of the peptide. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1.](http://images.novusbio.com/fullsize/PAK1-[p-Ser204]-Antibody-Western-Blot-NBP2-29665-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PAK1 [p Ser204] Antibody [NBP2-29665] - Untreated and pervanadate-treated NIH/3T3 cells (lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, phosphorylated-peptide blocked, or non-phosphorylated peptide blocked (lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1:1,000). Lanes 4 and 5 were blocking peptides were the phosphorylated peptide used as the immunogen and the non-phopshrylated form of the peptide. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1.")
