Recombinant Human PAG1 Protein

Product Discontinued

Recombinant Human PAG1 Protein Summary

• Description: Partial recombinant protein with His tag corresponding to the amino acids 41 - 432 of Human PAG1
  
  

  Source: Escherichia coli

  Amino Acid Sequence:

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PAG1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 10 mM Na2HPO4, 100 mM NaCl, pH 8
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >90%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with distilled water.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human PAG1 Protein

• CBP
• CBPMGC138364
• Csk-binding protein
• PAG1
• PAGFLJ37858
• phosphoprotein associated with glycosphingolipid microdomains 1
• phosphoprotein associated with glycosphingolipid-enriched microdomains 1
• Transmembrane adapter protein PAG
• Transmembrane phosphoprotein Cbp

Background

PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PAG1