PABPC4 Antibody

Western Blot: PABPC4 Antibody [NBP2-97931] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:71 kDaObserved band size:74 kDa

Immunocytochemistry/Immunofluorescence: PABPC4 Antibody [NBP2-97931] - Immunofluorescence staining of PABPC4 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human PABPC4 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunohistochemistry-Paraffin: PABPC4 Antibody [NBP2-97931] - Staining of human PABPC4 in human small intestine with rabbit polyclonal antibody at 1:2500 dilution.

Immunohistochemistry-Paraffin: PABPC4 Antibody [NBP2-97931] - Staining of human PABPC4 in human rectum with rabbit polyclonal antibody at 1:2500 dilution.

Immunoprecipitation: PABPC4 Antibody [NBP2-97931] - Lane A:0.5 mg U-251 MG Whole Cell Lysate1 uL anti-PABPC4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PABPC4 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 71 kDaObserved band size :75 kDa

Product Discontinued

PABPC4 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PABPC4 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PABPC4
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2500
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1-2 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.0)
• Preservative: 0.03% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for PABPC4 Antibody

• APP1FLJ43938
• APP-1PABP-4
• Inducible poly(A)-binding protein
• IPABP
• iPABPpoly(A)-binding protein, cytoplasmic 4 (inducible form)
• PABP4Activated-platelet protein 1
• poly(A) binding protein, cytoplasmic 4 (inducible form)
• Poly(A)-binding protein 4
• polyadenylate-binding protein 4

Background

Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PABPC4