p97/VCP Antibody

Images

 
Western Blot: p97/VCP Antibody [NB100-1557] - PC12 whole cell lysate, 30 ug total protein in each lane. Antibody at 1:1000. Image from verified customer review.
Immunohistochemistry: p97/VCP Antibody [NB100-1557] - Sample: FFPE sections of human ovarian carcinoma (upper) and mouse squamous cell carcinoma (lower). Antibody: Affinity purified rabbit anti-VCP used at a dilution of ...read more
Independent Antibodies: Western Blot: p97/VCP Antibody [NB100-1557] - Detection of Human and Mouse VCP on HeLa whole cell lysate using NB100-1557. VCP was also immunoprecipitated using rabbit anti-VCP antibodies ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ZeSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

p97/VCP Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415).
Predicted Species
Porcine (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VCP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000 - 1:15000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 1 - 4 ug/mg of lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
p97/VCP Lysate (NBL1-17707)
Reviewed Applications
Read 1 Review rated 5
using
NB100-1557 in the following applications:

Publications
Read Publications using
NB100-1557 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p97/VCP Antibody

  • 15S Mg(2+)-ATPase p97 subunit
  • IBMPFD
  • IBMPFDMGC8560
  • MGC131997
  • p97
  • TER ATPase
  • TERA
  • transitional endoplasmic reticulum ATPase
  • valosin containing protein
  • Valosin-containing proteinMGC148092
  • VCP
  • yeast Cdc48p homolog

Background

Valosin-containing protein (VCP) is a multifunctional protein that is proposed to be involved in vesicle transport and fusion for protein trafficking, 26S proteasome function in ubiquitin-based degradation pathways, and the assembly of peroxisomes. Mutations in the human VCP gene have been associated with diseases such as inclusion body myopathy, Paget disease of the bone, and frontotemporal dementia (IBMPFD). VCP can inhibit apoptosis and may play a role in tumorigenesis. VCP is also known as, Transitional Endoplasmic Reticulum ATPase, TER ATPase, TERA, 15S Mg (2+)-ATPase p97subunit, IBMPFD, Inclusion Body Myopathy associated with Paget Disease of Bone and Frontotemporal Dementia, MGC8560; MGC131997; MGC148092.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1557
Species: Hu, Mu, Rt, Po, Bv, Ze
Applications: WB, IHC, IHC-P, IP

Publications for p97/VCP Antibody (NB100-1557)(4)

Review for p97/VCP Antibody (NB100-1557) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB100-1557:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot p97/VCP NB100-1557
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reviewed by:
Luiz Alexandre Magno
WB Rat 10/17/2018
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Summary

ApplicationWestern Blot
Sample TestedPC12 cell whole cell lysate.
SpeciesRat
LotA1

Comments

CommentsIt works very well at 1:1,000 dilution when loading gels with 30 ug of protein extracts (PC12 cells in my case).

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Control Lysate(s)

Secondary Antibodies

 

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Diseases for p97/VCP Antibody (NB100-1557)

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Recent Reviews

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Luiz Alexandre Magno
10/17/2018
Application: WB
Species: Rat

Bioinformatics

Gene Symbol VCP
Entrez
Uniprot