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UFD1L Antibody (2A6F3)

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Western Blot: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis using UFD1L mAb against HEK293 (1) and UFD1L (AA: 208-307)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis using UFD1L mAb against HEK293 (1) and UFD1L (AA: 208-307)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis of Hela cells using UFD1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry-Paraffin: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis of endometrial cancer tissues using UFD1L mouse mAb with DAB staining.
Flow Cytometry: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis of Hela cells using UFD1L mouse mAb (green) and negative control (red).
Western Blot: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis using UFD1L mAb against human UFD1L (AA: 208-307) recombinant protein. (Expected MW is 36.8 kDa)
Western Blot: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis using UFD1L mouse mAb against K562 (1), Hela (2), A431 (3), PC-2 (4), and A549 (5) cell lysate.
Immunocytochemistry/ Immunofluorescence: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis of Hela cells using UFD1L mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 ...read more
Immunohistochemistry-Paraffin: UFD1L Antibody (2A6F3) [NBP2-61829] - Analysis of esophageal cancer tissues using UFD1L mouse mAb with DAB staining.
ELISA: UFD1L Antibody (2A6F3) [NBP2-61829] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2A6F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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UFD1L Antibody (2A6F3) Summary

Immunogen
Purified recombinant fragment of human UFD1L (AA: 208-307) expressed in E. Coli.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UFD1L
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
34.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for UFD1L Antibody (2A6F3)

  • ATP1C
  • ATP1G1ATPase, Na+/K+ transporting, gamma 1 polypeptide
  • FXYD domain containing ion transport regulator 2
  • FXYD domain-containing ion transport regulator 2
  • HOMG2
  • hypomagnesemia 2, renal
  • MGC12372
  • Na(+)/K(+) ATPase subunit gamma
  • Sodium pump gamma chain
  • sodium/potassium-transporting ATPase subunit gamma
  • Sodium-potassium-ATPase, gamma polypeptide

Background

The protein encoded by this gene forms a complex with two other proteins, nuclear protein localization-4 and valosin-containing protein, and this complex is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. A related pseudogene has been identified on chromosome 18.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (http://biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY licence.
Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1558 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1557. For blotting immunoprecipitated VCP, NB100-1558 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
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Species: Hu, Mu, Rt, Ze
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
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Western Blot: NPLOC4 Antibody [NBP1-82166] - Analysis using Anti-NPLOC4 antibody NBP1-82166 (A) shows similar pattern to independent antibody NBP1-82165 (B).
Immunocytochemistry/Immunofluorescence: NPLOC4 Antibody [NBP1-82166] - Staining of human cell line A-431 shows positivity in nuclei but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NPLOC4 Antibody [NBP1-82166] - Staining of human esophagus shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.
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Western Blot: Gemin 4 Antibody [NB110-40591] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NB110-40591 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry-Paraffin: Gemin 4 Antibody [NB110-40591] - Human breast carcinoma. Antibody used at a dilution of 1:200.
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Western Blot: Craniofacial Development Protein 1 Antibody (11811) [NBP2-43772] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CFDP1 antibody [11811].
Immunohistochemistry-Paraffin: Craniofacial Development Protein 1 Antibody (11811) [NBP2-43772] - Paraffin-embedded human ovarian cancer. CFDP1 stained by CFDP1 antibody [11811] diluted at 1:2000.
Western Blot: Craniofacial Development Protein 1 Antibody (11811) [NBP2-43772] - Analysis of A U87-MG whole cell lysate/extract B SK-N-SH whole cell lysate/extract C IMR32 whole cell lysate/extract D SK-N-AS whole cell lysate/extract 12 % SDS-PAGE CFDP1 antibody [11811] dilution: 1:1000
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Western Blot: Proteasome 19S 10B Antibody [NB100-1483] - Staining of HeLa (A), U251 (B), KNRK (C) with antibody at 1 ug/mL and NIH3T3 (D) cell lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody [NB100-1483] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: Proteasome 19S 10B Antibody [NB100-1483] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Western Blot: DAP5 Antibody (39C534.1) [NB120-11929] - Analysis of NB120-11929 (1ug) on 15 ug of total cell lysate from Jurkat cells.
Flow Cytometry: DAP5 Antibody (39C534.1) [NB120-11929] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Western Blot: NSFL1C Antibody [NBP2-13677] - Analysis using Anti-NSFL1C antibody NBP2-13677 (A) shows similar pattern to independent antibody NBP2-13676 (B).
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Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ING1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5758) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ING1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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Immunocytochemistry/Immunofluorescence: Pleckstrin Antibody [NB100-1343] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic/membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
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Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 cells and asCD11b-U937 cells in the absence or presence of 5nM TPA for 72 h, respectively, were 18-fold concentrated and subjected to SDS-PAGE containing 2 mg/ml of gelatine. Following incubation with MMP enzyme buffer, the gels were stained with 0.4% Coomassie blue and afterwards destained again to visualize the appearance of gelatinase activity by light bands against the dark background. The molecular weight markers on both sides of the gels indicate the size of the MMPs exhibiting gelatinase activities. Image collected and cropped by CiteAb from the following publication (http://biosignaling.biomedcentral.com/articles/10.1186/1478-811X-10-13) licensed under a CC-BY licence.
Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VCP antibody NB100-1558 used for IP at 3 ug per reaction. VCP was also immunoprecipitated by rabbit anti-VCP antibody NB100-1557. For blotting immunoprecipitated VCP, NB100-1558 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
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Western Blot: TBX1 Antibody (1C2) [NBP2-46076] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TBX1.
Immunohistochemistry: TBX1 Antibody (1C2) [NBP2-46076] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Immunocytochemistry/Immunofluorescence: CDC45L Antibody (JJ091-04) [NBP2-67897] - Staining CDC45 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CDC45L Antibody (JJ091-04) [NBP2-67897] - Analysis of paraffin-embedded mouse colon tissue using anti-CDC45 antibody. Counter stained with hematoxylin.
Flow Cytometry: CDC45L Antibody (JJ091-04) [NBP2-67897] - Analysis of 293 cells with CDC45 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Western Blot: AMFR/gp78 Antibody [NBP2-15374] - Sample (30 ug of whole cell lysate) A: K562 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: AMFR/gp78 Antibody [NBP2-15374] - Cal27 xenograft. AMFR antibody [N3C3] dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: AMFR/gp78 Antibody [NBP2-15374] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
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(2 Publications)
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Western Blot: HRD1 Antibody [NB100-2526] - Total protein from human HeLa, Hek293 and mouse 3T3 cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HRD1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.
Immunocytochemistry/Immunofluorescence: HRD1 Antibody [NB100-2526] - HRD1 antibody was tested in Hek293 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry-Paraffin: HRD1 Antibody [NB100-2526] - FFPE tissue section of human tonsil using rabbit polyclonal HRD1 antibody at 2 ug/mL concentration (1:500 dilution). The primary antibody binding to SYVN1/HRD1 antigen in cells was detected using HRP conjugated anti-Rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This HRD1 antibody generated an expected cytoplasmic staining of HRD1 with stronger signal around periphery of the nucleus signifying the staining of the endoplasmic reticulum.
(2 Reviews)
(11 Publications)
Species: Hu, Mu, Ha
Applications: WB, ICC/IF, IHC, IHC-P, KO

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Bioinformatics

Gene Symbol UFD1L
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