p63/TP73L Antibody (rTP40/3690)

Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human prostate stained with Biotinylated p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).

Flow Cytometry: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Flow Cytometric Analysis of PFA-fixed HeLa cells using p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).

Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human skin stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).

Immunohistochemistry-Paraffin: p63/TP73L Antibody (rTP40/3690) [NBP3-07430] - Formalin-fixed, paraffin-embedded human kidney stained with p63/TP73L Recombinant Mouse Monoclonal Antibody (rTP40/3690).

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, IHC-P
• Clone: rTP40/3690
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

p63/TP73L Antibody (rTP40/3690) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide from the N-terminal of human p63/TP73L protein (Exact sequence is proprietary)
• Localization: Nuclear
• Specificity: This antibody recognizes exclusively delta-Np63 but not TAp63. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung poorly differentiated adenocarcinoma. This antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, it may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma vs. invasive breast ductal carcinoma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TP63
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Notes

200ug/ml of antibody purified by Protein A Column. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08660)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for p63/TP73L Antibody (rTP40/3690)

• amplified in squamous cell carcinoma
• Chronic ulcerative stomatitis protein
• CUSP
• EEC3
• EEC3B(p51B)
• Keratinocyte transcription factor KET
• KET
• KETRHS
• LMS
• OFC8
• OFC8SHFM4TP73LNBP
• p40
• p51
• p51tumor protein p53-like
• p53CP
• p63
• p63AIS
• p73H
• p73L
• p73LB(p51A)
• SHFM4
• TP53CP
• TP53L
• TP63
• TP73L
• Transformation-related protein 63
• tumor protein 63
• tumor protein p53-competing protein
• tumor protein p63 deltaN isoform delta
• tumor protein p63
• Tumor protein p73-like

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP63