p53 Antibody

Images

 
Western Blot: p53 Antibody [NB200-171] - Detection of Human p53 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p53 ...read more
Immunohistochemistry: p53 Antibody [NB200-171] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti- p53 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Flow Cytometry: p53 Antibody [NB200-171] - p53 in Jurkat Cells, fixed in 1.5% PFA, and permeabilized in 90% Methanol. 1 million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 NB200-171 and secondary ...read more
Chromatin Immunoprecipitation (ChIP): p53 Antibody [NB200-171] - Chromatin from MCF7 was immunoprecipitated with anti-p53 antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of p53 ...read more
Western Blot: p53 Antibody [NB200-171] - Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody used at the indicated concentrations.
Flow Cytometry: p53 Antibody [NB200-171] - Baseline p53 expression in human lymphocytes. Image from verified customer review.
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BLM (NB100-324) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human BRD2 (NB100-260) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SMARCA4 (NBP2-22234) and rabbit anti-human p53 (). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-274) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate In Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms), FITC/Green (25ms) ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human DMAP1 (NB100-256) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HdmX (NBP2-22228) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human HP1-beta (NB100-79774) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MEN1 (NB100-287) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RAD17 (NB100-272) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RFC1 (NB100-230) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RPL26 (NBP2-22233) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SA1 (NB100-298) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms) and Texas Red ...read more
Proximity Ligation Assay: p53 Antibody [NB200-171] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human USP7 (NBP2-22295) and rabbit anti-human p53 (NB200-171). Image merged from DAPI (2ms), FITC/Green ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, Flow, IHC-P, IP, PLA, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

 

Genetic Strategies

   

p53 Antibody Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residues 50-100 of human tumor protein p53 using the SwissProt entry P04637 (GeneID 7157).
Localization
Cytoplasmic and nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TP53
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:25000
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-10 mg/mg
  • Proximity Ligation Assay 1:1000-1:20000
  • Immunofluorescence 1:500-1:2500
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
293 Lysate (NB800-PC6)
p53 Lysate (NBL1-17203)
Reviewed Applications
Read 1 Review rated 5
using
NB200-171 in the following applications:

Publications
Read Publications using
NB200-171 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies: NB100-305, NB100-322, NBP1-19098, NBP1-19097, NB100-376, NB100-1803, NB100-224, NB100-61615, NB100-77315, NB500-160, NB100-381, NB100-381, NB100-596, NB200-347, NB100-97835, NBP1-06527, NBP1-06515

Alternate Names for p53 Antibody

  • Antigen NY-CO-13
  • BCC7
  • FLJ92943
  • LFS1
  • LFS1TRP53
  • p53 tumor suppressor
  • p53
  • P53cellular tumor antigen p53
  • Phosphoprotein p53
  • TP53
  • transformation-related protein 53
  • TRP53
  • tumor protein p53
  • Tumor suppressor p53

Background

The tumor suppressor protein p53 plays an important role in cancer development. The p53 protein is a transcription factor that acts as a checkpoint in the cell cycle, either preventing or initiating programmed cell death. Since cancer occurs as the malfunction in the proliferation of cells, disrupting p53 activity allows abnormal cells to multiply or allows a tumor's progression from benign to malignant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p53 Antibody (NB200-171)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: FLOW.


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Review for p53 Antibody (NB200-171) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NB200-171:
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Flow
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Flow Cytometry p53 NB200-171
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reviewed by:
Anonymous
Flow Human 06/30/2014
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Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman

Primary Anitbody

Dilution Ratio1:100 dilution in BD Perm/Wash Buffer

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for p53 Antibody (NB200-171) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional p53 Products

Bioinformatics Tool for p53 Antibody (NB200-171)

Discover related pathways, diseases and genes to p53 Antibody (NB200-171). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for p53 Antibody (NB200-171)

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Pathways for p53 Antibody (NB200-171)

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PTMs for p53 Antibody (NB200-171)

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Research Areas for p53 Antibody (NB200-171)

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Blogs on p53. Showing 1-10 of 16 blog posts - Show all blog posts.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

PRMT6: One Function, Many Roles
Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

p14 ARF is an Important Tumor Suppressor
The p14ARF (Alternative Reading Frame) tumor suppressor is a protein product of the alternative reading frame (ARF) of the human INK4a locus which regulates a series of cell cycle regulatory proteins to promote cell cycle arrest in response to abnorma...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Recent Reviews

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1
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Anonymous
06/30/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol TP53
Entrez
Uniprot