P2Y12/P2RY12 Antibody

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Western Blot: P2Y12/P2RY12 Antibody [NBP1-78249] - Antibody dilution 1:500, MW is 41 kDa
Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Mouse brain, 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxlyin QS (blue) counterstain. 40X magnification, FFPE section.
Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat brain, 1:100 dilution.
Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat spinal cord. Dilution 1:100.
Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat spinal cord. Dilution 1:100.

Product Details

Summary
Reactivity Hu, Rt, PmSpecies Glossary
Applications WB, DB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

P2Y12/P2RY12 Antibody Summary

Immunogen
Synthetic peptide selected from human P2Y12 receptor gene corresponding to amino acids with the 320-342 at the C-terminus of protein. The peptide was covalently modified to achieve desired antigenicity before coupling to a carrier protein.
Specificity
This antibody detects a single 56-68kDa P2Y12 diffused band. The antibody does not cross reacts with other P2X or with other P2Y receptors on western blots.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
P2RY12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Dot Blot 1:10000
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:200
Application Notes
NBP1-78249 is ideal for WB and ELISA assays. Application in IHC, confocal and immpunoprecipitation protocol is not yet characterized.
Publications
Read Publication using NBP1-78249.

Reactivity Notes

Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for P2Y12/P2RY12 Antibody

  • ADPG-R
  • Gi-coupled ADP receptor HORK3
  • HORK3
  • HORK3P2Y12 platelet ADP receptor
  • P2RY12
  • P2T(AC)
  • P2Y purinoceptor 12
  • P2Y(AC)
  • P2Y(ADP)
  • P2Y(cyc)
  • P2Y12
  • P2Y12ADP-glucose receptor
  • purinergic receptor P2RY12
  • purinergic receptor P2Y, G-protein coupled, 12
  • putative G-protein coupled receptor
  • SP1999
  • SP1999G-protein coupled receptor SP1999

Background

Purine nucleotide receptor are classified in to two classes, the ionotrophic ligand-gated channels, P2X and the metabotropic G-protein coupled receptors, P2Y (1). There are 7 P2X receptors and 11 P2Y (P2Y1-2, 4, 6 and 11-14) each with unique agonist response profile has been identified. The subtypes of metabotropic receptors P2Y1 and P2Y2 are predominantly expressed in arterinal smooth muscle. P2Y receptors are G protein-coupled receptors (GPCRs) activated by nucleotides, such as ATP, ADP, UTP, UDP, or UDPglucose, depending on the receptor subtype. Currently, 8 subtypes are known including P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and P2Y14. P2Y receptors play an important role in osteoclastic resorption and ATP and UTP are inhibitors of bone formation in osteoblasts. Activation of P2 receptors in bone cells not only involves the propagation of intercellular calcium waves but also the proliferation and activity of the cells (2). Some of these receptors tend to associate with each other to make heterodimers. ADP is an endogenous agonist for both P2Y1 and P2Y12 on platelets, and antagonism of its action at P2Y1 or P2Y12 receptor has been shown to inhibit platelet aggregation. Adenine nucleotide antagonist, INS50589, binds to P2Y12 and has been used clinically in acute coronary syndrome patients. P2Y12 is a G-protein coupled receptor characterized of presence of 7TMD. The P2Y12 is negatively coupled via Gi to adenylate cyclase. Upon activation it inhibits the adenylyl cyclase second messenger system. Stimulation of P2 receptor releases a number of factor of inflammatory mediators like IL-1b, IL-1a, IL-6, IL-8 and TNFalpha (2). The P2Y12-receptor plays a crucial role in ADP-induced platelet aggregation. Both the P2Y1 and P2Y12 receptors contribute to platelet P-selectin exposure, interactions of platelets with leukocytes and exposure of tissue factor on activated leukocytes, which should translate into a direct role of these receptors in atherosclerosis (2).The P2Y12 is highly expressed in platelets, lower levels are reported in brain and lowest levels are detected in lung, appendix, pituitary and adrenal glands. The P2Y12 is also expressed in spinal cord and fetal brain. P2Y12 receptor when expressed in host cells showed significant heterogeneity on SDS-PAGE mobility like many other PGCRs. The P2Y12 is a 342 aa protein with characteristic 7TMDs of GPCR, exhibit a broad band od 52-60kDa. The P2Y12R sequence analyses suggest the presence of putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3rd intra cellular loop which interacts with G-proteins and has several phosphorylation sites. The P2Y12R-selective antibodies were generated against conserved but unique sequences on from P2Y12 protein form near C-terminal end form within 320-342 aa. The peptide sequence was conserved in human, monkey and some other species. The P2Y12R antibodies were affinity purified on an immobilized antigen based affinity chromatography. These epitope-specific antibodies strongly labeled P2Y12 protein in P2Y12-western blot positive controls. Anti-P2Y12-selective antibodies are also available as fluorescent conjugates for direct application in IHC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P2Y12/P2RY12 Antibody (NBP1-78249)(1)


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Bioinformatics

Gene Symbol P2RY12
Entrez
Uniprot