P2Y12/P2RY12 Antibodies
We offer P2Y12/P2RY12 Antibodies for use in common research applications: ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each P2Y12/P2RY12 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our P2Y12/P2RY12 Antibodies can be used in a variety of model species: Human, Primate. Use the list below to choose the P2Y12/P2RY12 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our P2Y12/P2RY12 polyclonal antibodies.
Alternate Names for P2Y12/P2RY12 Antibodies
anti-P2Y12/P2RY12 antibody, anti-P2RY12 antibody, anti-ADPG-R antibody, anti-Gi-coupled ADP receptor HORK3 antibody, anti-HORK3P2Y12 platelet ADP receptor antibody, anti-P2T(AC) antibody, anti-P2Y purinoceptor 12 antibody, anti-P2Y(AC) antibody, anti-P2Y(ADP) antibody, anti-P2Y(cyc) antibody, anti-P2Y12ADP-glucose receptor antibody, anti-purinergic receptor P2RY12 antibody, anti-purinergic receptor P2Y, G-protein coupled, 12 antibody, anti-putative G-protein coupled receptor antibody, anti-SP1999G-protein coupled receptor SP1999 antibody
9 Results for "p2y12-p2ry12" in Primary Antibodies
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to cell membrane of microglia. Protocol available in COMET™ Panel Builder." alt="P2Y12/P2RY12 was detected in immersion fixed paraffin-embedded sections of human Cortex using Rabbit Anti-human P2Y12/P2RY12, Polyclonal Antibody (Catalog # NBP2-33870) at 1:100 at 37°Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH).Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to cell membrane of microglia. Protocol available in COMET™ Panel Builder." class="big_thumb" />
![Western Blot: P2Y12/P2RY12 Antibody [NBP1-78249] - Antibody dilution 1:500, MW is 41 kDa](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Western-Blot-NBP1-78249-img0002.jpg "Western Blot: P2Y12/P2RY12 Antibody [NBP1-78249] - Antibody dilution 1:500, MW is 41 kDa")
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Mouse brain, 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxlyin QS (blue) counterstain. 40X magnification, FFPE section.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-78249-img0006.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat brain, 1:100 dilution.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-78249-img0003.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat spinal cord. Dilution 1:100.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-78249-img0004.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-78249] - Rat spinal cord. Dilution 1:100.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-78249-img0005.jpg)
![Western Blot: P2Y12/P2RY12 Antibody (1C2A9) [NBP2-61749] - Analysis using P2RY12 mAb against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-1C2A9-Western-Blot-NBP2-61749-img0004.jpg "Western Blot: P2Y12/P2RY12 Antibody (1C2A9) [NBP2-61749] - Analysis using P2RY12 mAb against HEK293 (1) and P2RY12 (AA: extra mix)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: P2Y12/P2RY12 Antibody (1C2A9) [NBP2-61749] - Analysis of Hela cells using P2RY12 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-1C2A9-Flow-Cytometry-NBP2-61749-img0002.jpg)
![Western Blot: P2Y12/P2RY12 Antibody (1C2A9) [NBP2-61749] - Analysis using P2RY12 mAb against human P2RY12 (AA: extra mix) recombinant protein. (Expected MW is 9.4 kDa)](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-1C2A9-Western-Blot-NBP2-61749-img0003.jpg)
![Western Blot: P2Y12/P2RY12 Antibody (1C2A9) [NBP2-61749] - Analysis using P2RY12 mouse mAb against PC-3 (1) and C6 (2) cell lysate.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-1C2A9-Western-Blot-NBP2-61749-img0005.jpg)
![ELISA: P2Y12/P2RY12 Antibody (1C2A9) [NBP2-61749] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-1C2A9-ELISA-NBP2-61749-img0001.jpg)
![Western Blot: P2Y12/P2RY12 Antibody [NBP3-35719] - Western blot analysis of lysates from Mouse brain using P2Y12/P2RY12 Rabbit pAb at 1:2000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25 ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-35719_rabbit-p2y12-p2ry12-pab-101220241233888.jpg "Western Blot: P2Y12/P2RY12 Antibody [NBP3-35719] - Western blot analysis of lysates from Mouse brain using P2Y12/P2RY12 Rabbit pAb at 1:2000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25 ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 45s.")
![Western Blot: P2Y12/P2RY12 Antibody [NBP3-35719] - Western Blot analysis of lysates from Mouse brain using P2Y12/P2RY12 Rabbit pAb at 1:2000 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25 ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Basic Kit.<br>Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-35719_rabbit-p2y12-p2ry12-pab-31120251744493.jpg)
![Immunocytochemistry/Immunofluorescence: P2Y12/P2RY12 Antibody [NLS3742] - Analysis of anti-P2RY12 / P2Y12 antibody with HEK293 human embryonic kidney cells](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunocytochemistry-NLS3742-img0007.jpg "Immunocytochemistry/Immunofluorescence: P2Y12/P2RY12 Antibody [NLS3742] - Analysis of anti-P2RY12 / P2Y12 antibody with HEK293 human embryonic kidney cells")
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NLS3742] - Ovary, Carcinoma](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NLS3742-img0012.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NLS3742] - Analysis of anti-P2RY12 / P2Y12 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NLS3742-img0006.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NLS3742] - Analysis of anti-P2RY12 / P2Y12 antibody with human blood, platelets.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NLS3742-img0008.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NLS3742] - Breast, Carcinoma](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NLS3742-img0010.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NLS3742] - Lung, Small Cell Carcinoma](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NLS3742-img0011.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-89673] - Staining in human cerebral cortex and skeletal muscle tissues using NBP1-89673 antibody. Corresponding P2RY12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-89673-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-89673] - Staining in human cerebral cortex and skeletal muscle tissues using NBP1-89673 antibody. Corresponding P2RY12 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-89673] - Staining of human cerebellum shows strong positivity in microglia.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-89673-img0005.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-89673] - Staining of human cerebral cortex shows strong positivity in microglia.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-89673-img0006.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-89673] - Staining of human lung shows moderate to strong membranous positivity in macrophages.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-89673-img0007.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP1-89673] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP1-89673-img0008.jpg)
![P2Y12/P2RY12 antibody [HL2829] detects P2Y12/P2RY12 protein at cell membrane by immunofluorescent analysis.</br>Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: P2Y12/P2RY12 stained by P2Y12/P2RY12 antibody [HL2829] (NBP3-48736) diluted at 1:500.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48736_rabbit-p2y12-p2ry12-mab-hl2829-immunocytochemistry-immunofluorescence-1411202518341519.jpg "P2Y12/P2RY12 antibody [HL2829] detects P2Y12/P2RY12 protein at cell membrane by immunofluorescent analysis.</br>Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: P2Y12/P2RY12 stained by P2Y12/P2RY12 antibody [HL2829] (NBP3-48736) diluted at 1:500.</br>Blue: Fluoroshield with DAPI .")
![<b>Orthogonal Strategies Validation. </b>Unboiled Non-transfected (-) and transfected (+) 293T and Expi293 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with P2Y12/P2RY12 antibody [HL2829] (NBP3-48736) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48736_rabbit-p2y12-p2ry12-mab-hl2829-western-blot-1411202518345918.jpg)
![Western Blot: P2Y12/P2RY12 Antibody (S02-2E1) [NBP3-19903] - Western blot detection of P2Y12/P2RY12 in A549 (Lane 1), HL-60 (Lane 2), U251 (Lane 3), U87-MG (Lane 4) cell lysates using NBP3-19903 (1:1000 diluted). Predicted band size: 40kDa. Observed band size: 52kDa.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-S02-2E1-Western-Blot-NBP3-19903-img0001.jpg "Western Blot: P2Y12/P2RY12 Antibody (S02-2E1) [NBP3-19903] - Western blot detection of P2Y12/P2RY12 in A549 (Lane 1), HL-60 (Lane 2), U251 (Lane 3), U87-MG (Lane 4) cell lysates using NBP3-19903 (1:1000 diluted). Predicted band size: 40kDa. Observed band size: 52kDa.")
![Western Blot: P2Y12/P2RY12 Antibody (SR1887) [NBP3-22528] - Western blot analysis using NBP3-22528 in RAW 264.7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22528_rabbit-p2y12-p2ry12-mab-sr1887-57202315521821.jpg "Western Blot: P2Y12/P2RY12 Antibody (SR1887) [NBP3-22528] - Western blot analysis using NBP3-22528 in RAW 264.7 cell lysate.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining in human cerebral cortex and liver tissues . Corresponding P2RY12 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0014.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human cerebral cortex shows strong cytoplasmic positivity in microglia.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0012.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human liver shows no positivity as expected.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0013.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0016.jpg)
![Immunohistochemistry-Paraffin: P2Y12/P2RY12 Antibody [NBP2-33870] - Staining of human cerebellum shows strong cytoplasmic positivity in microglia.](http://images.novusbio.com/fullsize/P2Y12-P2RY12-Antibody-Immunohistochemistry-Paraffin-NBP2-33870-img0017.jpg)
![Immunohistochemistry: P2Y12/P2RY12 Antibody [NBP2-33870] - Images demonstrating colocalization of CD105/MAB1097 with microglial markers (a) panels A, B Low magnification images of high-plaque (HP) case (panel A) & AD case (panel B) showing staining with CD105/MAB1097 (purple arrows) & IBA-1 (brown arrows) in MTG sections. Both images at same magnification: scale bars represent 100 μm. (b) (panel A) CD105 microglia (purple arrows) with ramified morphology stained with antibody to P2RY12 (brown arrow) in low-plaque (LP) case. (panel B) CD105 microglia (purple arrows) & HLA-DR (brown arrow) in AD case. (panel C) CD105 microglia (purple arrow) & CD45 (brown arrow) in AD case. All sections were from MTG. Images at same magnification: scale bars represent 50 μm. (c) (panel A–E). Patterns of staining of CD105 (purple) & IBA-1 (brown) in MTG sections of cases with progressively increasing pathology from LP to AD. (panel F). CD105-positive microglia in hippocampus (HPC) of AD case. Images at same magnification: scale bars represent 50 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31340569), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33870_rabbit-p2y12-p2ry12-pab-310202416114763.jpg)
