Western blot shows lysates of mouse platelets and rat platelets. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of rat platelets, loaded at 0.2 mg/mL. A specific band was detected for P‑Selectin/CD62P at approximately 197 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse ...read more
P‑Selectin/CD62P was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) at 5 µg/mL for 1 ...read more
Detects mouse P-Selectin/CD62P in direct ELISAs. Detects mouse and rat P-Selectin/CD62P in Western blot. In direct ELISAs, less than 15% cross-reactivity with recombinant human P-Selectin/CD62P is observed.
<0.10 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for P-Selectin/CD62P Antibody [Unconjugated]
selectin P (granule membrane protein 140kDa, antigen CD62)
Mouse P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).
P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2-terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.
Kansas, G.S. (1996) Blood 88:3259.
McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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