P-Selectin/CD62P Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse platelets and rat platelets. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of rat platelets, loaded at 0.2 mg/mL. A specific band was detected for P‑Selectin/CD62P at approximately 197 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse ...read more
P‑Selectin/CD62P was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) at 5 µg/mL for 1 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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P-Selectin/CD62P Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse P-Selectin/CD62P
Trp42-Ala709
Accession # Q01102
Specificity
Detects mouse P-Selectin/CD62P in direct ELISAs. Detects mouse and rat P-Selectin/CD62P in Western blot. In direct ELISAs, less than 15% cross-reactivity with recombinant human P-Selectin/CD62P is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SELP
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Simple Western 20 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publications using
AF737 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Selectin/CD62P Antibody [Unconjugated]

  • CD62P antigen
  • CD62P
  • FLJ45155
  • GMP140
  • GRMP
  • PADGEM
  • PADGEMantigen CD62)
  • PSEL
  • P-Selectin
  • selectin P (granule membrane protein 140kDa, antigen CD62)
  • SELP

Background

Mouse P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2-terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.

  1. Kansas, G.S. (1996) Blood 88:3259.
  2. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P-Selectin/CD62P Antibody (AF737)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Functional Assay, ICC, IHC, IHC Paraffin-embedded.


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Functional Assay
(1)
ICC
(2)
IHC
(2)
IHC Paraffin-embedded
(1)
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Human
(1)
Mouse
(4)
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Showing Publications 1 - 6 of 6.
Publications using AF737 Applications Species
AP Fournier, A Quenault, S Martinez d, M Gauberti, G Defer, D Vivien, F Docagne, R Macrez Prediction of disease activity in models of multiple sclerosis by molecular magnetic resonance imaging of P-selectin Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28533365] (IHC,, Mouse) IHC Mouse
A Mizrachi, Y Shamay, J Shah, S Brook, J Soong, VK Rajasekhar, JL Humm, JH Healey, SN Powell, J Baselga, DA Heller, A Haimovitz-, M Scaltriti Tumour-specific PI3K inhibition via nanoparticle-targeted delivery in head and neck squamous cell carcinoma Nat Commun, 2017;8(0):14292. 2017 [PMID: 28194032] (IHC,, Mouse) IHC Mouse
Aurélien Quenault Molecular magnetic resonance imaging discloses endothelial activation after transient ischaemic attack Brain, 2017;140(0):146-157. 2017 [PMID: 28031221] (Functional Assay, ) Functional Assay
Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC, Vlasuk GP, Pittman DD Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis. Atherosclerosis, 2008;0(0):. 2008 [PMID: 18377911] (ICC,, Mouse) ICC Mouse
Tikellis C, Jandeleit-Dahm KA, Sheehy K, Murphy A, Chin-Dusting J, Kling D, Sebokova E, Cooper ME, Mizrahi J, Woollard KJ Reduced plaque formation induced by rosiglitazone in an STZ-diabetes mouse model of atherosclerosis is associated with downregulation of adhesion molecules. Atherosclerosis, 2008;199(1):55-64. 2008 [PMID: 18093596] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Washington AV, Schubert RL, Quigley L, Disipio T, Feltz R, Cho EH, McVicar DW A TREM family member, TLT-1, is found exclusively in the alpha-granules of megakaryocytes and platelets. Blood, 2004;104(4):1042-7. 2004 [PMID: 15100151] (ICC,, Human) ICC Human

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Bioinformatics

Gene Symbol SELP
Uniprot