P-Selectin/CD62P Antibody [Unconjugated]

Images

 
Western blot shows lysates of human platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF137) followed by ...read more
Western blot shows lysates of rat platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF137) followed by ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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P-Selectin/CD62P Antibody [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human P‑Selectin/CD62P
Specificity
Detects human and rat P-Selectin/CD62P in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse P-Selectin, and less than 1% cross-reactivity with recombinant human (rh) E-Selectin and rhL-Selectin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SELP
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Adhesion Blockade
Publications
Read Publications using
AF137 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Selectin/CD62P Antibody [Unconjugated]

  • CD62P antigen
  • CD62P
  • FLJ45155
  • GMP140
  • GRMP
  • PADGEM
  • PADGEMantigen CD62)
  • PSEL
  • P-Selectin
  • selectin P (granule membrane protein 140kDa, antigen CD62)
  • SELP

Background

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin,P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for P-Selectin/CD62P Antibody (AF137)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: ELISA Development, Flow, ICC, Neut, WB.


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ELISA Development
(1)
Flow
(1)
ICC
(1)
Neut
(2)
WB
(1)
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Human
(6)
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Showing Publications 1 - 6 of 6.
Publications using AF137 Applications Species
NL Stevenson, IJ White, JJ McCormack, C Robinson, DF Cutler, TD Nightingal Clathrin-mediated post-fusion membrane retrieval influences the exocytic mode of endothelial Weibel-Palade bodies J. Cell. Sci., 2017;0(0):. 2017 [PMID: 28674075] (ICC,, Human) ICC Human
Poeter M, Brandherm I, Rossaint J, Rosso G, Shahin V, Skryabin B, Zarbock A, Gerke V, Rescher U Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63. Nat Commun, 2014;5(0):3738. 2014 [PMID: 24769558] (Flow,, Human) Flow Human
Stevenson N, Martin-Martin B, Freeman J, Kriston-Vizi J, Ketteler R, Cutler D G protein-coupled receptor kinase 2 moderates recruitment of THP-1 cells to the endothelium by limiting histamine-invoked Weibel-Palade body exocytosis. J Thromb Haemost, 2014;12(2):261-272. 2014 [PMID: 24735118] (Neut,, Human) Neut Human
Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX, 2011;28(4):305-16. 2011 [PMID: 22130485] (WB,, Human) WB Human
Kato N, Yuzawa Y, Kosugi T The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. J. Am. Soc. Nephrol., 2009;20(7):1565-76. 2009 [PMID: 19443639] (Neut,, Human) Neut Human
Choudhury A, Chung I, Blann AD, Lip GY Elevated platelet microparticle levels in nonvalvular atrial fibrillation: relationship to p-selectin and antithrombotic therapy. Chest, 2007;131(3):809-15. 2007 [PMID: 17356097] (ELISA Development,, Human) ELISA Development Human

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Bioinformatics

Gene Symbol SELP