P-Selectin/CD62P Antibody (AK-4) - BSA Free

Flow Cytometry: P-Selectin/CD62P Antibody (AK-4) [NBP1-43392] - Staining of thrombin-activated human peripheral blood platelets with 0.5 ug of Mouse IgG1 kappa Isotype Control Purifed (open histogram) or 0.5 ug of Anti-Human CD62P (P-Selectin) Purified (filled histogram) followed by Anti-Mouse IgG FITC. Total viable cells were used for analysis.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: AK-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

P-Selectin/CD62P Antibody (AK-4) - BSA Free Summary

• Additional Information: Clone AK-4 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was CD62P.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
• Application Notes: This AK-4 antibody has been tested by flow cytometric analysis of resting and thrombin-activated human platelets. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for P-Selectin/CD62P Antibody (AK-4) - BSA Free

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

The AK-4 monoclonal antibody reacts with human CD62P, a 140 kDa transmembrane glycoprotein also known as P-Selectin. Upon activation, CD62P is rapidly transported from its cytoplasmic stores in the Weibel-Palade bodies of endothelial cells or the alpha-granules of platelets to the surface. CD62P initiates the adhesive interaction between endothelial cells and neutrophils and monocytes during the inflammatory reaction and is also involved in the interaction of platelets with monocytes and neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P-Selectin/CD62P Antibody (NBP1-43392)(2)

Publications using NBP1-43392

• Sousa HR, Gaspar RS, Sena EM et al. Novel antiplatelet role for a protein disulfide isomerase-targeted peptide: Evidence of covalent binding to C-terminal CGHC redox motif J. Thromb. 2017-01-21 [PMID: 28109047] (FLOW, Human)
  
  Details:
  This citation used the PerCP version of this antibody.
• Skinner MP, Lucas CM, Burns GF et al. GMP-140 binding to neutrophils is inhibited by sulfated glycans J Biol Chem 1991-01-01 [PMID: 1706335]
  
  Details:
  This citation used the PE version of this antibody.

Bioinformatics

• Gene Symbol: SELP