P-Selectin/CD62P Antibody (AK-4) [FITC]

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: AK-4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

P-Selectin/CD62P Antibody (AK-4) [FITC] Summary

• Additional Information: Clone AK-4 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was CD62P.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for P-Selectin/CD62P Antibody (AK-4) [FITC]

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P-Selectin/CD62P Antibody (NBP1-43392F)(1)

Publication using NBP1-43392F

• Sousa HR, Gaspar RS, Sena EM et al. Novel antiplatelet role for a protein disulfide isomerase-targeted peptide: Evidence of covalent binding to C-terminal CGHC redox motif J. Thromb. 2017-01-21 [PMID: 28109047] (FLOW, Human)
  
  Details:
  This citation used the PerCP version of this antibody.

Bioinformatics

• Gene Symbol: SELP