OXCT1 Antibody - BSA Free

Immunohistochemistry-Paraffin: OXCT1 Antibody [NBP1-82462] - Analysis in human heart muscle and liver tissues. Corresponding OXCT1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: OXCT1 Antibody [NBP1-82462] - Staining of human heart muscle, kidney, liver and testis using Anti-OXCT1 antibody NBP1-82462 (A) shows similar protein distribution across tissues to independent antibody NBP2-49331 (B).

Western Blot: OXCT1 Antibody [NBP1-82462] - Analysis in human cell lines HeLa and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Western Blot: OXCT1 Antibody [NBP1-82462] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/Immunofluorescence: OXCT1 Antibody [NBP1-82462] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: OXCT1 Antibody [NBP1-82462] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: OXCT1 Antibody [NBP1-82462] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.

Immunohistochemistry-Paraffin: OXCT1 Antibody [NBP1-82462] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

OXCT1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit OXCT1 Antibody - BSA Free (NBP1-82462) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-OXCT1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: VGGFGLCGIPENLIDALLKTGVKGLTAVSNNAGVDNFGLGLLLRSKQIKRMVSSYVGENAEFERQYLSGELEVELTPQGTLAERIRAGGAGVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OXCT1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  OXCT1 Recombinant Protein Antigen (NBP1-82462PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for OXCT1 Antibody - BSA Free

• 3-oxoacid CoA transferase 1,3-oxoacid CoA transferase
• EC 2.8.3
• EC 2.8.3.5
• SCOTmitochondrial

Background

OXCT1 encodes a member of the 3-oxoacid CoA-transferase gene family. The encoded protein is a homodimeric mitochondrial matrix enzyme that plays a central role in extrahepatic ketone body catabolism by catalyzing the reversible transfer of coenzyme A from succinyl-CoA to acetoacetate. Mutations in this gene are associated with succinyl CoA:3-oxoacid CoA transferase deficiency. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OXCT1 Antibody (NBP1-82462)(3)

Publications using NBP1-82462

• Gouirand V, Gicquel T, Lien EC et al. Ketogenic HMG-CoA lyase and its product beta-hydroxybutyrate promote pancreatic cancer progression The EMBO journal 2022-03-21 [PMID: 35307861] (WB, IF/IHC, Mouse)
• Ding J, Li H, Liu Y Et al. OXCT1 Enhances Gemcitabine Resistance Through NF-kappaB Pathway in Pancreatic Ductal Adenocarcinoma Frontiers in oncology 2021-11-05 [PMID: 34804914]
• Chang HT, Olson LK, Schwartz KA. Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy. Nutr Metab (Lond) 2013-07-05 [PMID: 23829383]

Bioinformatics

• Gene Symbol: OXCT1
• Entrez:
  • 5019(Human)
• Uniprot:
  • P55809(Human)