NNT Antibody

Images

 
Western Blot: NNT Antibody [NBP1-32109] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: NNT Antibody [NBP1-32109] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Paraffin-embedded mouse kidney dilution: 1:500.
Western Blot: NNT Antibody [NBP1-32109] - (30 ug of whole cell lysate) A:A431 B:H1299.
Western Blot: NNT Antibody [NBP1-32109] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug HeLa whole cell lysate/extract, C. 30 ug HepG2 whole cell lysate/extract |. D. 30 ug A37C whole cell lysate/extract 7. 5 % ...read more
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Paraffin-embedded mouse stomach dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.86 mg/ml

Order Details

NNT Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human NNT. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane, Multi-pass membrane protein, Matrix side
Predicted Species
Bovine (99%), Xenopus (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NNT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Zebrafish (88%), Xenopus laevis (93%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.86 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NNT Antibody

  • MGC126502
  • mitochondrial
  • nicotinamide nucleotide transhydrogenaseMGC126503
  • Pyridine nucleotide transhydrogenase

Background

This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional NNT Products

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol NNT