NNT Antibody


Western Blot: NNT Antibody [NBP1-32109] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:500. ...read more
Immunocytochemistry/ Immunofluorescence: NNT Antibody [NBP1-32109] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NNT protein stained by NNT antibody diluted at 1:500. Blue: Hoechst 33342 ...read more
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Paraffin-embedded mouse kidney dilution: 1:500.
Western Blot: NNT Antibody [NBP1-32109] - A. 30 ug 293T whole cell lysate/extract, B. 30 ug HeLa whole cell lysate/extract, C. 30 ug HepG2 whole cell lysate/extract |. D. 30 ug A37C whole cell lysate/extract 7. 5 % ...read more
Western Blot: NNT Antibody [NBP1-32109] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NNT antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-32109] - Paraffin-embedded mouse stomach dilution: 1:500.

Product Details

Reactivity Hu, Mu, Rt, Bv, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P

Order Details

NNT Antibody Summary

Recombinant protein encompassing a sequence within the C-terminus region of human NNT. The exact sequence is proprietary.
Mitochondrion inner membrane, Multi-pass membrane protein, Matrix side
Predicted Species
Bovine (99%), Xenopus (93%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (88%), Xenopus laevis (93%).

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 10% Glycerol
0.01% Thimerosal
Immunogen affinity purified

Alternate Names for NNT Antibody

  • MGC126502
  • mitochondrial
  • nicotinamide nucleotide transhydrogenaseMGC126503
  • Pyridine nucleotide transhydrogenase


This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Gene Symbol NNT