Otx2 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC207479 and based on accession number NM_021728. The protein contains a C-terminal DDK tag.
Homo sapiens orthodenticle homeobox 2 (OTX2), transcript variant 1, mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for Otx2
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Otx2 Overexpression Lysate (Native)
- homeobox protein OTX2
- orthodenticle homeobox 2
- orthodenticle homolog 2 (Drosophila)
- Orthodenticle homolog 2
This gene encodes a member of the bicoid sub-family of homeodomain-containing transcription factors. The encoded protein acts as a transcription factor and may play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants may exist, but their full length sequences have not been determined. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript, and encodes the longer isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Applications: WB, ELISA, ICC/IF, RNAi
Applications: WB, IHC, IHC-P, PEP-ELISA
Species: Hu, Mu, Rt, Mk, Pm, Sh
Applications: WB, ICC/IF, IP
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
Applications: WB, IHC
Species: Hu, Mu
Applications: WB, ChIP, ELISA, ICC/IF
Applications: WB, ELISA, ICC/IF, IHC-P
Applications: IHC, IHC-P
Species: Hu, Ca, Pm
Species: Hu, Mu, Rt
Species: Hu, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Applications: WB, ChIP, ICC
Applications: WB, ELISA, IHC-P
Applications: WB, IHC, IHC-P
Applications: WB, ELISA, ICC/IF
Publications for Otx2 Lysate (NBL1-14015) (0)
There are no publications for Otx2 Lysate (NBL1-14015).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Otx2 Lysate (NBL1-14015) (0)
There are no reviews for Otx2 Lysate (NBL1-14015).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Otx2 Lysate (NBL1-14015) (0)
Additional Otx2 Products
Bioinformatics Tool for Otx2 Lysate (NBL1-14015)
Discover related pathways, diseases and genes to Otx2 Lysate (NBL1-14015). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on Otx2