OTUD7B/Cezanne/ZA20D1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: OTUD7B/Cezanne/ZA20D1 Antibody [NBP2-97842] - Immunofluorescence staining of OTUD7B/Cezanne/ZA20D1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human OTUD7B/Cezanne/ZA20D1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

OTUD7B/Cezanne/ZA20D1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human OTUD7B/Cezanne/ZA20D1 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OTUD7B
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for OTUD7B/Cezanne/ZA20D1 Antibody - BSA Free

• cellular zinc finger NF-kappaB Cezanne
• Cellular zinc finger NF-kappa-B protein
• Cezanne
• CEZANNEA20 domain containing 1
• EC 3.4.19.12
• OTU domain containing 7B
• OTU domain-containing protein 7B
• OTUD7B
• ZA20D1
• Zinc finger A20 domain-containing protein 1
• Zinc finger protein Cezanne

Background

ZA20D1 has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains.Hydrolyzes both linear and branched forms of polyubiquitin. Negative regulator of nuclear factor NF-kappa-B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OTUD7B