CLC Antibody (138815) [Unconjugated]

CLC was detected in immersion fixed paraffin-embedded sections of human prostate using Mouse Anti-Human CLC Monoclonal Antibody (Catalog # MAB962) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 138815
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CLC Antibody (138815) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CLC
  Leu28-Phe225
  Accession # Q9UBD9
• Specificity: Detects human CLC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) CT-1, rhCNTF, rhIL-6, rhIL-11, rhLIF, or rhOSM is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLCF1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CLC Antibody (138815) [Unconjugated]

• B-cell stimulating factor 3
• B-cell stimulatory factor 3
• BSF-3
• BSF3BSF-3
• cardiotrophin-like cytokine factor 1
• CISS2
• CLC
• CLCF1
• CLCNNT-1
• CRLF1 associated cytokine-like factor 1
• neurotrophin-1/B-cell stimulating factor-3
• NNT-1
• NNT1B-cell-stimulating factor 3
• Novel neurotrophin-1
• NR6

Background

Cardiotrophin-like cytokine (CLC) (also known as novel neurotrophin-1 (NNT-1) and B cell stimulating factor (BSF-3)) is a recently discovered 22-25 kDa member of the IL-6 family of cytokines (1, 2, 3). As such, it is expressed as a long type I cytokine with four alpha -helices in it structure (2). Human CLC is synthesized as a 225 amino acid precursor that contains a 27 aa signal sequence and a 198 aa mature region. It contains one potential N-linked glycosylation site that is apparently utilized, and two distinct binding sites for CNTFR alpha and CLF (4, 5). Although CLC has a signal sequence, it is not secreted unless noncovalently dimerized to either CLF or soluble CNTFR alpha (5, 6). Once dimerized, CLC signals through a tripartite receptor complex composed of gp130, LIFR beta and CNTFR alpha (membrane-bound) (5, 7). Within the IL-6 family, human CLC is most homologous to cardiotrophin-1, sharing approximately 29% amino acid sequence identity (8).  Human to mouse, mature CLC is 96% aa identical. CLC is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation (9, 10, 11). Cells known to express CLC include embryonic muscle, lung epithelium, and mesenchyme in various regions (12).

1. Murakami, M. et al. (2004) Growth Factors 22:75.
2. Ernst, M. & B.J. Jenkins (2004) Trends Genet. 20:23.
3. Senaldi, G. et al. (1999) Proc.Natl. Acad. Sci. USA 96:11458.
4. Perret, D. et al. (2003) J. Biol. Chem. 279:43961.
5. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.
6. Elson, G.C.A. et al. (2000) Nat. Neurosci. 3:867.
7. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
8. Derouet, D. et al. (2004) Proc. Natl. Acad. Sci. USA 101:4827.
9. Forger, N.G. et al. (2003) J. Neurosci. 23:8854.
10. Auernhammer, C.J. et al. (2003) Endocrinology 144:1202.
11. Senaldi, G. et al. (2002) J. Immunol. 168:5690.
12. de Bovis, B. et al. (2005) Cell Commun. Signal. 3:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CLC Antibody (MAB962)(1)

Publication using MAB962

• S Pasquin, S Chehboun, A Dejda, Y Meliani, V Savin, GJ Warner, R Bosse, A Tormo, G Mayer, M Sharma, P Sapieha, C Martel, JF Gauchat Effect of human very low-density lipoproteins on cardiotrophin-like cytokine factor 1 (CLCF1) activity Sci Rep, 2018-03-05;8(1):3990. 2018-03-05 [PMID: 29507344] (Immunoprecipitation, Western Blot)

Bioinformatics

• Gene Symbol: CLCF1
• Uniprot:
  • Q9UBD9()