| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88095-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35742-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17748-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97842-50ul | |||
![Western Blot: OTUD7B/Cezanne/ZA20D1 Antibody [NBP1-88095] - COP1 & OTUD7B coordinate to control NPCs differentiation. a, b NPCs were plated in Matrigel-coated six-well plate for neuronal differentiation. For the indicated times, immunoblotting (a) & immunofluorescence (b) of Sox2, CUL4A, COP1, OTUD7B & TUJ1 were performed. c Cells transfected with HA-Ub & indicated shRNA were treated by MG132 for 8 h, & the lysates were immunoprecipated with anti-Sox2 antibody & immunoblotted with anti-HA. d Summary of our findings. OTUD7B stabilizes Sox2 through deubiquitylation & maintains the stemness of NPCs, while CUL4ADET1-COP1 promotes Sox2 degradation through ubiquitylation & induces differentiation of NPCs. OTUD7B & CUL4ADET1-COP1 exert opposite roles in regulating Sox2 protein stability at the post-translational level. The representative images are shown from three independent experiments. Unprocessed original scans of blots are shown in Supplementary Fig. 9 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30405104), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-88095_rabbit-polyclonal-otud7b-cezanne-za20d1-antibody-310202416114710.jpg)
![Immunohistochemistry-Paraffin: OTUD7B/Cezanne/ZA20D1 Antibody [NBP1-88095] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/OTUD7B-Cezanne-ZA20D1-Antibody-Immunohistochemistry-Paraffin-NBP1-88095-img0010.jpg)
![Immunohistochemistry: OTUD7B/Cezanne/ZA20D1 Antibody [NBP3-35742] - Immunohistochemistry analysis of paraffin-embedded Human colon using OTUD7B/Cezanne/ZA20D1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35742_rabbit-otud7b-cezanne-za20d1-pab-101220241136020.jpg "Immunohistochemistry: OTUD7B/Cezanne/ZA20D1 Antibody [NBP3-35742] - Immunohistochemistry analysis of paraffin-embedded Human colon using OTUD7B/Cezanne/ZA20D1 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Western Blot: OTUD7B/Cezanne/ZA20D1 Antibody [NBP3-35742] - Western blot analysis of various lysates using OTUD7B/Cezanne/ZA20D1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-35742_rabbit-otud7b-cezanne-za20d1-pab-101220241134331.jpg)
![Immunocytochemistry/ Immunofluorescence: OTUD7B/Cezanne/ZA20D1 Antibody [NBP3-35742] - Immunofluorescence analysis of L929 cells using OTUD7B/Cezanne/ZA20D1 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35742_rabbit-otud7b-cezanne-za20d1-pab-1012202411353086.jpg)
![Immunocytochemistry/Immunofluorescence: OTUD7B/Cezanne/ZA20D1 Antibody (2B4) [H00056957-M01] - Analysis of monoclonal antibody to OTUD7B on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/OTUD7B-Cezanne-ZA20D1-Antibody-2B4-Immunocytochemistry-Immunofluorescence-H00056957-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: OTUD7B/Cezanne/ZA20D1 Antibody (2B4) [H00056957-M01] - Analysis of monoclonal antibody to OTUD7B on HeLa cell . Antibody concentration 10 ug/ml.")
![Immunohistochemistry-Paraffin: OTUD7B/Cezanne/ZA20D1 Antibody (2B4) [H00056957-M01] - Analysis of monoclonal antibody to OTUD7B on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/OTUD7B-Cezanne-ZA20D1-Antibody-2B4-Immunohistochemistry-Paraffin-H00056957-M01-img0004.jpg)
![Sandwich ELISA: OTUD7B/Cezanne/ZA20D1 Antibody (2B4) [H00056957-M01] - Detection limit for recombinant GST tagged OTUD7B is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/OTUD7B-Cezanne-ZA20D1-Antibody-2B4-Sandwich-ELISA-H00056957-M01-img0005.jpg)
![Western Blot: OTUD7B/Cezanne/ZA20D1 Antibody [NBP2-87998] - WB Suggested Anti-OTUD7B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate](http://images.novusbio.com/fullsize/OTUD7B-Cezanne-ZA20D1-Antibody-Western-Blot-NBP2-87998-img0002.jpg "Western Blot: OTUD7B/Cezanne/ZA20D1 Antibody [NBP2-87998] - WB Suggested Anti-OTUD7B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: 293T cell lysate")
![Immunohistochemistry-Paraffin: OTUD7B/Cezanne/ZA20D1 Antibody [NBP2-87998] - Rabbit Anti-OTUD7B Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult heart. Observed Staining: Cytoplasmic. Primary Antibody Concentration: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy2/3.](http://images.novusbio.com/fullsize/OTUD7B-Cezanne-ZA20D1-Antibody-Immunohistochemistry-Paraffin-NBP2-87998-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: OTUD7B/Cezanne/ZA20D1 Antibody [NBP3-17748] - Staining of human cell line SiHa shows localization to microtubules, cytokinetic bridge & mitotic spindle.](http://images.novusbio.com/fullsize/OTUD7B-Cezanne-ZA20D1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17748-img0001.jpg "Immunocytochemistry/Immunofluorescence: OTUD7B/Cezanne/ZA20D1 Antibody [NBP3-17748] - Staining of human cell line SiHa shows localization to microtubules, cytokinetic bridge & mitotic spindle.")
![Western Blot: OTUD7B/Cezanne/ZA20D1 Antibody [NBP2-87997] - WB Suggested Anti-ZA20D1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Daudi cell lysateOTUD7B is supported by BioGPS gene expression data to be expressed in Daudi](http://images.novusbio.com/fullsize/OTUD7B-Cezanne-ZA20D1-Antibody-Western-Blot-NBP2-87997-img0001.jpg "Western Blot: OTUD7B/Cezanne/ZA20D1 Antibody [NBP2-87997] - WB Suggested Anti-ZA20D1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Daudi cell lysateOTUD7B is supported by BioGPS gene expression data to be expressed in Daudi")
![Western Blot: ZA20D1 Overexpression Lysate (Adult Normal) [NBL1-14013] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZA20D1.](http://images.novusbio.com/fullsize/ZA20D1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14013-img0002.jpg "Western Blot: ZA20D1 Overexpression Lysate (Adult Normal) [NBL1-14013] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ZA20D1.")
![Immunocytochemistry/Immunofluorescence: OTUD7B/Cezanne/ZA20D1 Antibody [NBP2-97842] - Immunofluorescence staining of OTUD7B/Cezanne/ZA20D1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97842 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97842_rabbit-polyclonal-otud7b-cezanne-za20d1-antibody-23520251628129.png "Immunocytochemistry/Immunofluorescence: OTUD7B/Cezanne/ZA20D1 Antibody [NBP2-97842] - Immunofluorescence staining of OTUD7B/Cezanne/ZA20D1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97842 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")