ZRANB1/Trabid Antibody

Images

 
Western Blot: ZRANB1/Trabid Antibody [NBP1-01469] - Staining of HumanCerebellum lysate(35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.1ug/ml)
Western Blot: ZRANB1/Trabid Antibody [NBP1-01469] - Analysis of ZRANB1 in 293T whole cell lysate transfected with HA-Zranb1 plasmid using anti-ZRANB1 antibody. Image from verified customer review.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ZRANB1/Trabid Antibody Summary

Immunogen
Peptide with sequence KWLDRYRQIRPCTS corresponding to C-Terminus according to NP_060050.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ZRANB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 80 kDa band bands observed in human brain (cerebellum) lysates (calculated MW of 81.0 kDa band according to NP_060050.2).
Control
ZRANB1/Trabid Lysate (NBP2-65374)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-01469 in the following applications:

Publications
Read Publication using NBP1-01469.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZRANB1/Trabid Antibody

  • HRPE773
  • jacalin-like lectin domain containing 2
  • JCLN2
  • pancreatic adenocarcinoma upregulated factor
  • PAUF
  • PRO1567
  • Trabid
  • ZRANB1
  • zymogen granule protein 16 homolog B (rat)
  • zymogen granule protein 16 homolog B

Background

Positive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. Acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription. May also modulate TNF-alpha signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ZRANB1/Trabid Antibody (NBP1-01469)(1)

Review for ZRANB1/Trabid Antibody (NBP1-01469) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-01469:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot ZRANB1/Trabid NBP1-01469
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reviewed by:
Anonymous
WB Human 07/14/2015
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Summary

ApplicationWestern Blot
Sample Tested293T whole cell lysate transfected with HA-Zranb1 plasmid
SpeciesHuman

Blocking

Blocking Details5% MILK, 30 MIN, ROOM TEMPERATURE

Primary Anitbody

Dilution Ratio1:5000, OVERNIGHT, 4 DEGREE, 5% MILK

Secondary Antibody

Secondary Description1:5000 ANTI GOAT, 1 hour, ROOM TEMPERATURE, 5% MILK

Details

Detection NotesSuperSignal™ West Dura Extended Duration Substrate, 1-2 MINUTES. ALSO CONFIRMED BY USING HA ANTIBODY.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ZRANB1/Trabid Antibody (NBP1-01469) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for ZRANB1/Trabid Antibody (NBP1-01469)

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Diseases for ZRANB1/Trabid Antibody (NBP1-01469)

Discover more about diseases related to ZRANB1/Trabid Antibody (NBP1-01469).
 

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Recent Reviews

5
5
1
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2
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1
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Anonymous
07/14/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol ZRANB1
Entrez
OMIM
Uniprot