ZRANB1/Trabid Antibody

ZRANB1/Trabid Antibody [NBP1-01469] - (1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ZRANB1/Trabid Antibody Summary

• Description: Novus Biologicals Goat ZRANB1/Trabid Antibody (NBP1-01469) is a polyclonal antibody validated for use in WB and ELISA. Anti-ZRANB1/Trabid Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence KWLDRYRQIRPCTS corresponding to C-Terminus according to NP_060050.2.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ZRANB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.5-2ug/ml
• Application Notes: WB: Approx. 80 kDa band bands observed in human brain (cerebellum) lysates (calculated MW of 81.0 kDa band according to NP_060050.2). An additional band of unknown identity was also consistently observed at 38kDa.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ZRANB1/Trabid Antibody

• HRPE773
• jacalin-like lectin domain containing 2
• JCLN2
• pancreatic adenocarcinoma upregulated factor
• PAUF
• PRO1567
• Trabid
• ZRANB1
• zymogen granule protein 16 homolog B (rat)
• zymogen granule protein 16 homolog B

Background

Positive regulator of the Wnt signaling pathway that specifically cleaves 'Lys-63'-linked ubiquitin chains. Acts by deubiquitinating APC protein, a negative regulator of Wnt-mediated transcription. May also modulate TNF-alpha signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ZRANB1/Trabid Antibody (NBP1-01469)(1)

Publication using NBP1-01469

• Tran H, Hamada F, Schwarz-Romond T, Bienz M. Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains. Genes Dev 2008-02-15 [PMID: 18281465]

Review for ZRANB1/Trabid Antibody (NBP1-01469) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 07/14/2015

Summary

• Application: Western Blot
• Sample Tested: 293T whole cell lysate transfected with HA-Zranb1 plasmid
• Species: Human

Bioinformatics

• Gene Symbol: ZRANB1
• Entrez:
  • 54764(Human)
  • 360216(Mouse)
• OMIM:
  • 54764(Human)
• Uniprot:
  • Q9UGI0(Human)