Osteoprotegerin/TNFRSF11B Antibody (98A1071)

Images

 
Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - IHC analysis of a formalin fixed paraffin embedded tissue section of human liver using 1:200 dilution of ...read more
Western Blot: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Total protein from human MG63 and Daudi cell lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% ...read more
Immunocytochemistry/ Immunofluorescence: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - MG-63 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% ...read more
Flow Cytometry: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Intracellular staining of 10^6 HeLa cells using 0.2 ug of OPG antibody, 0.5 ul of PE secondary.
Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Formalin-fixed, paraffin-embedded normal human breast tissue stained with OPG antibody (5 ug/ml), peroxidase-conjugate and DAB ...read more
Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. ...read more
Immunohistochemistry-Paraffin: Osteoprotegerin/TNFRSF11B Antibody (98A1071) [NB100-56505] - Staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
98A1071
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Osteoprotegerin/TNFRSF11B Antibody (98A1071) Summary

Immunogen
A peptide corresponding to the amino acids 20-37 TQETFPPKYLHYDEETSH of human Osteoprotegerin was used as immunogen for this antibody.
Specificity
The Osteoprotegerin antibody, clone 98A1071, detects all forms of Osteoprotegerin including monomeric, dimeric and Osteoprotegerin bound to RANKL (Vandooren, 2008) as well as Osteoprotegerin bound to TRAIL (Sandra, 2006).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF11B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:20-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:20-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:20-1:1000
  • CyTOF-ready
Application Notes
This antibody is useful in Flow Cytometry: 0.2-1 ug/10^6 cells Immunocytochemistry/Immunofluorescence: see Sandra 2006 Immunohistochemistry (frozen): see Boumans 2012 for details. Immunoprecipitation: see Sandra 2006
Publications
Read Publications using
NB100-56505 in the following applications:

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 11028444)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Osteoprotegerin/TNFRSF11B Antibody (98A1071)

  • OCIF
  • OCIFMGC29565
  • OPGtumor necrosis factor receptor superfamily member 11B
  • Osteoclastogenesis inhibitory factor
  • Osteoprotegerin
  • TNFRSF11B
  • TR1
  • tumor necrosis factor receptor superfamily, member 11b

Background

Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor (TNFR) superfamily. As the name implies, it protects bone. Unlike other TNFRs it lacks a transmembrane domain and lacks any apparent cell associated signals. OPG consists of 401 amino acid with molecular mass of approximately 55 kD as a monomer and 110 kD as a disulfide-linked dimer. High levels of OPG mRNA has been detected in lung, heart, kidney, and placenta1. Recombinant OPG blocks osteoclastogenesis in vitro and increases bone density in vivo. Targeted deletion of OPG in mice results in severe early-onset of osteoporosity. These mice also exhibit increase in vascular calcification in the aorta and renal arteries suggesting that it may also play a role in degenerative arterial disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for Osteoprotegerin/TNFRSF11B Antibody (NB100-56505)(27)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 5 applications: ELISA, ICC/IF, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(1)
IHC-Fr
(1)
IHC-P
(4)
WB
(2)
All Applications
Filter By Species
Human
(5)
Rat
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NB100-56505 Applications Species
Nozawa S, Inubushi T, Irie F, Takigami I. Osteoblastic heparan sulfate regulates osteoprotegerin function and bone mass. JCI Insight. 2018 Feb 08 [PMID: 29415886] (ICC/IF, Human) ICC/IF Human
Scimeca M, Salustri A, Bonanno E et al. Impairment of PTX3 expression in osteoblasts: a key element for osteoporosis. Cell Death Dis. 2017 Oct 12 [PMID: 29022895] (Human) Human
Stanislawowski M, Wisniewski P, Guzek M et al. Influence of receptor activator of nuclear factor kappa B ligand, osteoprotegerin and interleukin-33 on bone metabolism in patients with long-standing ulcerative colitis. J Crohns Colitis. 2014 Jan 15 [PMID: 24439762] (IHC-Fr, Human)

Details:
Colonic mucosa biopsies, Fig 7C
IHC-Fr Human
Larson SR, Zhang X, Dumpit R et al. Characterization of osteoblastic and osteolytic proteins in prostate cancer bone metastases. Prostate. 2013 Jun [PMID: 23334979]
Boumans MJ, Thurlings RM, Yeo L et al. Rituximab abrogates joint destruction in rheumatoid arthritis by inhibiting osteoclastogenesis. Ann Rheum Dis. 2012 Jan [PMID: 22072013]
Witasp Anna, Carrero Juan J, Hammarqvist Folke et al. Expression of osteoprotegerin in human fat tissue; implications for chronic kidney disease. Eur J Clin Invest. 2011 May [PMID: 21128937] (Human) Human
Tomlins Christine, Storey Alan. Cutaneous HPV5 E6 causes increased expression of Osteoprotegerin and Interleukin 6 which contribute to evasion of UV-induced apoptosis. Carcinogenesis. 2010 Dec [PMID: 20884685] (WB) WB
Pohjolainen V, Taskinen P, Soini Y et al. Noncollagenous bone matrix proteins as a part of calcific aortic valve disease regulation. Hum Pathol. 2008 Nov [PMID: 18701137]
Vandooren B, Cantaert T, Noordenbos T et al. The abundant synovial expression of the RANK/RANKL/Osteoprotegerin system in peripheral spondylarthritis is partially disconnected from inflammation. Arthritis Rheum. 2008 Mar [PMID: 18311801]
Waterman EA, Cross NA, Lippitt JM et al. The antibody MAB8051 directed against osteoprotegerin detects carbonic anhydrase II: implications for association studies with human cancers. Int J Cancer. 2007 Nov 1 [PMID: 17631639]
Show All 27 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Osteoprotegerin/TNFRSF11B Antibody (NB100-56505). (Showing 1 - 1 of 1 FAQs).

  1. Are the following monoclonals purified from ascites or from tissue culture supernatant?NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712
    • These antibodies are all purified from tissue culture supernatant.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56505AF405
Alexa Fluor 488 Labeled NB100-56505AF488
Alexa Fluor 647 Labeled NB100-56505AF647
Alexa Fluor 700 Labeled NB100-56505AF700
Allophycocyanin Labeled NB100-56505APC
Biotin Labeled NB100-56505B
DyLight 350 Labeled NB100-56505UV
DyLight 405 Labeled NB100-56505V
DyLight 488 Labeled NB100-56505G
DyLight 550 Labeled NB100-56505R
DyLight 650 Labeled NB100-56505C
DyLight 680 Labeled NB100-56505FR
DyLight 755 Labeled NB100-56505IR
HRP Labeled NB100-56505H
PE Labeled NB100-56505PE
PerCP Labeled NB100-56505PCP

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Bioinformatics

Gene Symbol TNFRSF11B
Entrez
Uniprot