Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

Analysis in human cerebral cortex and tonsil tissues using NBP3-44052 antibody. Corresponding Osteopontin/OPN RNA-seq data are presented for the same tissues.

Staining of human cerebellum shows moderate cytoplasmic positivity in microglia.

Staining of human cerebral cortex (Alzheimer disease) shows moderate cytoplasmic positivity in microglia.

Staining of human kidney shows strong cytoplasmic positivity in cells in distal tubules.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

• Reactivity: Hu
• Applications: IHC
• Clone: CL10686
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free (NBP3-07980) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P10451, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Osteopontin/OPN Antibody (CL10686) - Azide and BSA Free

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPP1