ORP150/HSP12A Antibody

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Western Blot: ORP150/HSP12A Antibody [H00010525-B01P] - Analysis of HYOU1 expression in human liver.
Western Blot: ORP150/HSP12A Antibody [H00010525-B01P] - Analysis of HYOU1 expression in transfected 293T cell line by HYOU1 polyclonal antibody. Lane 1: HYOU1 transfected lysate(74.58 KDa). Lane 2: Non-transfected ...read more

Product Details

Summary
Product Discontinued
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    • Catalog Number
      H00010525-B01P
    • Availability
      Product Discontinued

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ORP150/HSP12A Antibody Summary

Immunogen
HYOU1 (AAH72436.1, 1 a.a. - 678 a.a.) full-length human protein. MADKVRRQRPRRRVCWALVAVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPVIVTLKENERFFGDSAASMAIKNPKATLRYFQHLLGKQADNPHVALYQARFPEHELTFDPQRQTVHFQISSQLQFSPEEVLGMVLNYSRSLAEDFAEQPIKDAVITVPVFFNQAERRAVLQAARMAGLKVLQLINDNTATALSYGVFRRKDINTTAQNIMFYDMGSGSTVCTIVTYQMVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLRERLAGLFNEQRKGQRAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRVEFEELCADLFERVPGPVQQALQSAEMSLDEIEQVILVGGATRVPRVQEVLLKAVGKEELGKNINADEAAAMGAVYQAAALSKAFKVKPFVVRDAVVYPILVEFTREVEEEPGIHSLKHNKRVLFSRMGPYPQRKVITFNRYSHDFNFHINYGDLGFLGPEDLRVFGSQNLTTVKLKGVGDSFKKYPDYESKGIKAHFNLDESGVLSLDRVESVFETLVEDSAEEESTLTKLGNTISSLFGGGTTPDAKENGTDTVQEEEESPAEGSKDEPGEQVELKEEAEAPVEDGSQPPPPEPKGDATPEGEKATEKENGDKSEAQKPSVCRASVTRFGF
Specificity
HYOU1 - hypoxia up-regulated 1,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
HYOU1
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500
Application Notes
Antibody reactive against transfected lysate and tissue lysate for Western Blot. Has also been used for ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ORP150/HSP12A Antibody

  • 150 kDa oxygen-regulated protein
  • 170 kDa glucose-regulated protein
  • DKFZp686N08236
  • FLJ97572
  • glucose-regulated protein 170
  • Grp170
  • GRP-170
  • HSP12A
  • HYOU1
  • hypoxia up-regulated 1
  • hypoxia up-regulated protein 1
  • ORP150
  • ORP-150
  • ORP150FLJ94899
  • oxygen regulated protein (150kD)

Background

The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment is found at the 5' end of the first exon, and is involved in stress-dependent induction. A transcript that is produced from a downstream start site is preferentially induced by hypoxia, resulting in the accumulation of this protein in the endoplasmic reticulum (ER). The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol HYOU1
Entrez
Uniprot